Table 1. PCR primer sequences and... | American Society of Hematology

Table 1.

PCR primer sequences and GenBank accession numbers

Primer name	Forward primer	Reverse primer	Length, bp	Accession no.	Ta, °C
CD11b	TGTGGACATGGACGCTGATG	GCCTATGATCCGGTGGCTGT	331	NM_008401	60
CD18	GCCGAGTGCCTGAAGTTTGAT	TAAGCCACGTTGTGTTTGCAGA	504	X14951	60
C/EBPα	GCCCCTCAGTCCCTGTCTTTAG	ATGGTCCCCGTGTCCTCCTA	302	BC051102	60
C/EBPϵ	CAGCTGGACTCAGAAGACAGGC	ACAGCTTCCCTTGTCCAGGTG	306	XM_139135	60
GM-CSF2Rβ2	ACCTCCAATCCTCAACCAGACC	CTGTACCCATAAACACGGCCAA	378	NM_007781	60
GM-CSFRα	ACGGAGGTCACAAGGTCAAGGT	ACACGCCCACTTTGGTGATTG	454	M85078	60
GM-CSFRβ1	ATCCAGCTCATGGTGCCACTT	TCACCACCGGAGAGCATTTCT	520	NM_007780	60
EPOR	ATCTCGTTGTTGCTGACGGTTC	TCCAATACCAAGTAGGTGTCCTGG	332	BC046282	60
c-FMS	AACCCTGGCTACTCCTCCATCC	GCTTCATCCTACTCGGACCCCT	301	BC039758	60
G-CSFR	CAAACTGGACCAACAGGCAAA	GGAGCAGTTGTTCTGCCTCTTC	406	M58288	60
GATA-1	TAAGGTGGCTGAATCCTCTGCATC	CCGTCTTCAAGGTGTCCAAGAACGT	445	NM_008089	58
GATA-2	TTCTTCTGCAGGGGGTAGTGTAG	GGTGACTTCTCTTGCATGCACTT	693	NM-008090	58
GM-CSF	TGCAGACCCGCCTGAAGATAT	ATACTGCCCTCCAACTGTGGCT	353	X03019	60
Gr-1	CCTTCCAGGAACCCTGTACTGA	TGCTGTCCATATCCATGAGCA	370	XM_194966	60
HPRT	GCTGGTGAAAAGGACCTCTC	ATGGCCACAGGACTAGAACAC	254	J00423	62
IL-6R	GTTCTACAGAAGCAACGAGTGTCCTC	AGTCTCTGTTGCTGTTGTCATAAGG	336	XM_194097	56
c-MPL	ATGCCTACCGAGGAGAGAAGCC	GCCATAGCGGAGTTCATGCC	317	NM_010823	60

Primer name	Forward primer	Reverse primer	Length, bp	Accession no.	Ta, °C
CD11b	TGTGGACATGGACGCTGATG	GCCTATGATCCGGTGGCTGT	331	NM_008401	60
CD18	GCCGAGTGCCTGAAGTTTGAT	TAAGCCACGTTGTGTTTGCAGA	504	X14951	60
C/EBPα	GCCCCTCAGTCCCTGTCTTTAG	ATGGTCCCCGTGTCCTCCTA	302	BC051102	60
C/EBPϵ	CAGCTGGACTCAGAAGACAGGC	ACAGCTTCCCTTGTCCAGGTG	306	XM_139135	60
GM-CSF2Rβ2	ACCTCCAATCCTCAACCAGACC	CTGTACCCATAAACACGGCCAA	378	NM_007781	60
GM-CSFRα	ACGGAGGTCACAAGGTCAAGGT	ACACGCCCACTTTGGTGATTG	454	M85078	60
GM-CSFRβ1	ATCCAGCTCATGGTGCCACTT	TCACCACCGGAGAGCATTTCT	520	NM_007780	60
EPOR	ATCTCGTTGTTGCTGACGGTTC	TCCAATACCAAGTAGGTGTCCTGG	332	BC046282	60
c-FMS	AACCCTGGCTACTCCTCCATCC	GCTTCATCCTACTCGGACCCCT	301	BC039758	60
G-CSFR	CAAACTGGACCAACAGGCAAA	GGAGCAGTTGTTCTGCCTCTTC	406	M58288	60
GATA-1	TAAGGTGGCTGAATCCTCTGCATC	CCGTCTTCAAGGTGTCCAAGAACGT	445	NM_008089	58
GATA-2	TTCTTCTGCAGGGGGTAGTGTAG	GGTGACTTCTCTTGCATGCACTT	693	NM-008090	58
GM-CSF	TGCAGACCCGCCTGAAGATAT	ATACTGCCCTCCAACTGTGGCT	353	X03019	60
Gr-1	CCTTCCAGGAACCCTGTACTGA	TGCTGTCCATATCCATGAGCA	370	XM_194966	60
HPRT	GCTGGTGAAAAGGACCTCTC	ATGGCCACAGGACTAGAACAC	254	J00423	62
IL-6R	GTTCTACAGAAGCAACGAGTGTCCTC	AGTCTCTGTTGCTGTTGTCATAAGG	336	XM_194097	56
c-MPL	ATGCCTACCGAGGAGAGAAGCC	GCCATAGCGGAGTTCATGCC	317	NM_010823	60