PSAC activity does not depend on energy or phosphorylation status
Agent . | Mechanism of action . |
|---|---|
| Glucose, 10 mM | Energy source |
| Na3VO4, 1 mM and 5 mM | Inhibitor of ATPases and kinases |
| Sp-cAMPS, 10 μM | cAMP-dependent kinase agonist |
| Rp-cAMPS, 20 μM | cAMP-dependent kinase antagonist |
| Okadaic acid, 5 μM | Protein phosphatase inhibitor |
| Theophylline, 100 μM | Phosphodiesterase inhibitor |
| Isoproterenol, 20 μM | β-adrenergic receptor agonist |
| Staurosporine, 3 μM | Nonspecific protein kinase inhibitor |
Agent . | Mechanism of action . |
|---|---|
| Glucose, 10 mM | Energy source |
| Na3VO4, 1 mM and 5 mM | Inhibitor of ATPases and kinases |
| Sp-cAMPS, 10 μM | cAMP-dependent kinase agonist |
| Rp-cAMPS, 20 μM | cAMP-dependent kinase antagonist |
| Okadaic acid, 5 μM | Protein phosphatase inhibitor |
| Theophylline, 100 μM | Phosphodiesterase inhibitor |
| Isoproterenol, 20 μM | β-adrenergic receptor agonist |
| Staurosporine, 3 μM | Nonspecific protein kinase inhibitor |
Each agent was tested for its effects on infected and uninfected RBCs using the osmotic lysis assay after a 15-minute preincubation period at the indicated concentrations. Glucose and Na3VO4 were also evaluated and found to have no effect in single-channel patch-clamp studies.