T-cell development
. | . | . | . | CD3+, % . | . | |
|---|---|---|---|---|---|---|
| Mouse . | Construct . | CD45+, % . | GFP+ in CD45+, % . | GFP+ . | GFP− . | |
| 1 | p53i | 80.3 | 6.6 | 25.2 | 22.1 | |
| 2 | p53i | 60.0 | 66.0 | 97.2 | 94.8 | |
| 3 | p53i | 99.4 | 94.2 | 87.7 | 96.3 | |
| 4 | p53i | 97.5 | 60.6 | 99.4 | 94.0 | |
| 5 | p53i | 97.4 | 25.1 | 56.7 | 33.2 | |
| 6 | p53i | 97.6 | 85.9 | 68.3 | 85.8 | |
| 7 | p53i | 98.5 | 29.0 | 98.7 | 53.6 | |
| 8 | Control | 45.2 | 5.2 | 25.9 | 32.4 | |
| 9 | Control | 63.0 | 36.5 | 97.4 | 95.5 | |
| 10 | Control | 99.0 | 99.0 | 95.7 | 76.0 | |
. | . | . | . | CD3+, % . | . | |
|---|---|---|---|---|---|---|
| Mouse . | Construct . | CD45+, % . | GFP+ in CD45+, % . | GFP+ . | GFP− . | |
| 1 | p53i | 80.3 | 6.6 | 25.2 | 22.1 | |
| 2 | p53i | 60.0 | 66.0 | 97.2 | 94.8 | |
| 3 | p53i | 99.4 | 94.2 | 87.7 | 96.3 | |
| 4 | p53i | 97.5 | 60.6 | 99.4 | 94.0 | |
| 5 | p53i | 97.4 | 25.1 | 56.7 | 33.2 | |
| 6 | p53i | 97.6 | 85.9 | 68.3 | 85.8 | |
| 7 | p53i | 98.5 | 29.0 | 98.7 | 53.6 | |
| 8 | Control | 45.2 | 5.2 | 25.9 | 32.4 | |
| 9 | Control | 63.0 | 36.5 | 97.4 | 95.5 | |
| 10 | Control | 99.0 | 99.0 | 95.7 | 76.0 | |
Newborn mice were injected with CD34+ fetal liver cells transduced with p53i siRNA-GFP or control-GFP constructs. Mice were killed, and thymi were analyzed using FACS. The percentages of CD3+ cells in the GFP+ and GFP− gates are given. Percentages of CD45+ cells and the proportion of GFP+ cells in this gate are also indicated.