Detection of TTAGGG repeats in chromatid breaks and fusions in clastogen-treated Fancg−/− cells by Q- FISH analysis
. | Chromatid breaks . | . | Chromatid fusions . | . | ||
|---|---|---|---|---|---|---|
| Treatment . | With TTAGGG at the break . | Without TTAGGG at the break . | With TTAGGG at the fusion point . | Without TTAGGG at the fusion point . | ||
| Clastogen | ||||||
| Splenocytes | ||||||
| Nontreated | 0 | 0.3 | 0 | 0 | ||
| MMC, 10 ng/mL | 0 | 3.9 | 0 | 0.05 | ||
| MEFs | ||||||
| Nontreated | 0 | 0.07 | 0 | 0 | ||
| MMC, 15 ng/mL | 0 | 1.4 | 0 | 0.53 | ||
| γ-irradiation | ||||||
| Splenocytes | ||||||
| Nontreated | 0 | 0.1 | 0 | 0 | ||
| 2 Gy | 0 | 0.38 | 0 | 0.03 | ||
| 4 Gy | 0 | 0.38 | 0 | 0.08 | ||
| BMCs | ||||||
| Nontreated | 0 | 0.1 | 0 | 0 | ||
| 2 Gy | 0 | 0.1 | 0 | 0 | ||
| 4 Gy | 0 | 0.92 | 0 | 0.36 | ||
| MEFs | ||||||
| Nontreated | 0 | 0.06 | 0 | 0 | ||
| 2 Gy | 0 | 0.23 | 0 | 0.08 | ||
. | Chromatid breaks . | . | Chromatid fusions . | . | ||
|---|---|---|---|---|---|---|
| Treatment . | With TTAGGG at the break . | Without TTAGGG at the break . | With TTAGGG at the fusion point . | Without TTAGGG at the fusion point . | ||
| Clastogen | ||||||
| Splenocytes | ||||||
| Nontreated | 0 | 0.3 | 0 | 0 | ||
| MMC, 10 ng/mL | 0 | 3.9 | 0 | 0.05 | ||
| MEFs | ||||||
| Nontreated | 0 | 0.07 | 0 | 0 | ||
| MMC, 15 ng/mL | 0 | 1.4 | 0 | 0.53 | ||
| γ-irradiation | ||||||
| Splenocytes | ||||||
| Nontreated | 0 | 0.1 | 0 | 0 | ||
| 2 Gy | 0 | 0.38 | 0 | 0.03 | ||
| 4 Gy | 0 | 0.38 | 0 | 0.08 | ||
| BMCs | ||||||
| Nontreated | 0 | 0.1 | 0 | 0 | ||
| 2 Gy | 0 | 0.1 | 0 | 0 | ||
| 4 Gy | 0 | 0.92 | 0 | 0.36 | ||
| MEFs | ||||||
| Nontreated | 0 | 0.06 | 0 | 0 | ||
| 2 Gy | 0 | 0.23 | 0 | 0.08 | ||
All numbers are aberrations per metaphase.