Immunolocalization of B-B4 in human normal tissues
. | . | Frozen section . | . | Paraffin (1st sample Biochain, 2nd Zymed) . |
|---|---|---|---|---|
| Tissue . | No. cases . | Intensity*cell type or structure . | No. cases . | Intensity*cell type or structure . |
| Cardiovascular | ||||
| Heart | 2 | 0/myocardium | 2 | 0/myocardium |
| Respiratory | ||||
| Lung | 1 | 0/epithelial, endothelial cells, alveoli; 3 scattered/macrophages | 1 | 0/alveoli, scattered macrophages |
| 1 | 0/epithelial cells; 3 scattered/macrophages | 1 | 0/alveoli | |
| 1 | 0/epithelium, endothelial cells, alveoli | ND | ||
| 1 | 0/endothelium; 0/alveoli, bronchiole epithelium not present | ND | ||
| Gastrointestinal | ||||
| Esophagus | 1 | 3 hetero/outer squamous epithelium; 0/submucosa, muscularis | 1 | 0/muscularis (no squamous epithelium present) |
| 1 | 3 homo/stratified squamous epithelium; 0/submucosa, muscularis | 1 | 3 homo/stratified squamous epithelium; 0/muscularis | |
| Stomach | 1 | 0 | 1 | 0-1 focal/epithelial cells |
| ND | 1 | 2 scattered/cells, possibly parietal cells | ||
| Jejunum | ND | 1 | 3 homo/surface epithelium; 3/plasma cells | |
| Ileum | ND | 1 | 2 hetero/epithelial cells; 0/smooth muscle | |
| Colon | 1 | 3 homo/surface epithelium; 0/submucosa, muscularis | 1 | 1 hetero/epithelium; 3/scattered cells |
| ND | 1 | 3 homo/epithelium | ||
| Rectum | ND | 1 | 0 | |
| Pancreas | ND | 1 | 0 | |
| ND | 1 | 3 scattered/cells lining ducts | ||
| Liver | 1 | 3 hetero/granular staining around hepatocytes, appears nonspecific | 1 | 3 homo/sinusoidal lining cells; 0/hepatocytes |
| 1 | 2 hetero/granular staining around hepatocytes, appears nonspecific | 1 | 2 hetero/sinusoidal lining cells; 0/hepatocytes | |
| 1 | 1 homo/background | ND | ||
| 1 | 2 hetero/possibly sinusoidal lining cells | ND | ||
| Gall bladder | ND | 1 | 0/no epithelium | |
| Genitourinary | ||||
| Kidney | 1 | 0/glomeruli; 3 homo/tubules (cortex) | 1 | 0/glomeruli; 1 homo/tubules |
| 1 | 0/glomeruli; 3 homo/tubules (cortex) | 1 | 0/glomeruli; 0/tubules | |
| 1 | 0/glomeruli; 2 homo/tubules (cortex) | ND | ||
| 1 | 3 homo/tubules, glomeruli not present (medulla) | ND | ||
| Bladder | ND | 1 | 0/smooth muscle, no epithelium present | |
| Cervix | ND | 1 | 0/smooth muscle, no epithelium present | |
| ND | 1 | 0/connective tissue; 3 homo/squamous mucosa | ||
| Testis | ND | 1 | 0 | |
| ND | 1 | 0 | ||
| Ovary | ND | 1 | 0 | |
| ND | 1 | 0 | ||
| Uterus | ND | 1 | 0 | |
| ND | 1 | 1 homo/epithelium | ||
| Miscellaneous | ||||
| Adipose | ND | 1 | 0 | |
| Brain | 1 | 0 | 1 | 0 |
| ND | 1 | 0/cerebrum; 0/cerebellum | ||
| Breast | 1 | 2 homo/ductal epithelium; 2 focal/connective tissue | 1 | 2 hetero/ductal epithelium |
| ND | 1 | 0/ductal epithelium | ||
| Bone marrow | 1 | 0/most cells; 3 scattered/cells | 1 | 0 |
| Diaphragm | ND | 1 | 0 | |
| Placenta | ND | 1 | 3 focal/cells lining trophoblasts | |
| ND | 1 | 3 scattered/cells lining trophoblasts | ||
| Skeletal muscle | ND | 1 | 0 | |
| ND | 1 | 2 hetero | ||
| Skin | 2 | 0/dermis; 3 homo/epidermis | 2 | 0/dermis; 3 hetero/epidermis, appendages |
| Spleen | 2 | 0/lymphoid; 3 scattered/cells, macrophages | 2 | 0/lymphoid |
| Thymus | 1 | 2 hetero/Hassall corpuscle; 3 focal/possibly medulla | 1 | 0/lymphoid; 3 scattered/plasma cells |
| ND | 1 | 0/lymphoid | ||
| Thyroid | ND | 1 | 0/follicle epithelium | |
| ND | 1 | 3 scattered/plasma cells | ||
| Tonsil | ND | 2 | 0/lymphoid; 3 hetero/squamous mucosa, scattered plasma cells | |
| Umbilical cord | 1 | 0/vessels are negative | ND | |
| OPM-2 cells | ||||
| Positive CNT | 1 | 3 homo | 1 | 3 homo |
| Namaiwa cells | ||||
| Negative CNT | 1 | 0 | 1 | 0 |
. | . | Frozen section . | . | Paraffin (1st sample Biochain, 2nd Zymed) . |
|---|---|---|---|---|
| Tissue . | No. cases . | Intensity*cell type or structure . | No. cases . | Intensity*cell type or structure . |
| Cardiovascular | ||||
| Heart | 2 | 0/myocardium | 2 | 0/myocardium |
| Respiratory | ||||
| Lung | 1 | 0/epithelial, endothelial cells, alveoli; 3 scattered/macrophages | 1 | 0/alveoli, scattered macrophages |
| 1 | 0/epithelial cells; 3 scattered/macrophages | 1 | 0/alveoli | |
| 1 | 0/epithelium, endothelial cells, alveoli | ND | ||
| 1 | 0/endothelium; 0/alveoli, bronchiole epithelium not present | ND | ||
| Gastrointestinal | ||||
| Esophagus | 1 | 3 hetero/outer squamous epithelium; 0/submucosa, muscularis | 1 | 0/muscularis (no squamous epithelium present) |
| 1 | 3 homo/stratified squamous epithelium; 0/submucosa, muscularis | 1 | 3 homo/stratified squamous epithelium; 0/muscularis | |
| Stomach | 1 | 0 | 1 | 0-1 focal/epithelial cells |
| ND | 1 | 2 scattered/cells, possibly parietal cells | ||
| Jejunum | ND | 1 | 3 homo/surface epithelium; 3/plasma cells | |
| Ileum | ND | 1 | 2 hetero/epithelial cells; 0/smooth muscle | |
| Colon | 1 | 3 homo/surface epithelium; 0/submucosa, muscularis | 1 | 1 hetero/epithelium; 3/scattered cells |
| ND | 1 | 3 homo/epithelium | ||
| Rectum | ND | 1 | 0 | |
| Pancreas | ND | 1 | 0 | |
| ND | 1 | 3 scattered/cells lining ducts | ||
| Liver | 1 | 3 hetero/granular staining around hepatocytes, appears nonspecific | 1 | 3 homo/sinusoidal lining cells; 0/hepatocytes |
| 1 | 2 hetero/granular staining around hepatocytes, appears nonspecific | 1 | 2 hetero/sinusoidal lining cells; 0/hepatocytes | |
| 1 | 1 homo/background | ND | ||
| 1 | 2 hetero/possibly sinusoidal lining cells | ND | ||
| Gall bladder | ND | 1 | 0/no epithelium | |
| Genitourinary | ||||
| Kidney | 1 | 0/glomeruli; 3 homo/tubules (cortex) | 1 | 0/glomeruli; 1 homo/tubules |
| 1 | 0/glomeruli; 3 homo/tubules (cortex) | 1 | 0/glomeruli; 0/tubules | |
| 1 | 0/glomeruli; 2 homo/tubules (cortex) | ND | ||
| 1 | 3 homo/tubules, glomeruli not present (medulla) | ND | ||
| Bladder | ND | 1 | 0/smooth muscle, no epithelium present | |
| Cervix | ND | 1 | 0/smooth muscle, no epithelium present | |
| ND | 1 | 0/connective tissue; 3 homo/squamous mucosa | ||
| Testis | ND | 1 | 0 | |
| ND | 1 | 0 | ||
| Ovary | ND | 1 | 0 | |
| ND | 1 | 0 | ||
| Uterus | ND | 1 | 0 | |
| ND | 1 | 1 homo/epithelium | ||
| Miscellaneous | ||||
| Adipose | ND | 1 | 0 | |
| Brain | 1 | 0 | 1 | 0 |
| ND | 1 | 0/cerebrum; 0/cerebellum | ||
| Breast | 1 | 2 homo/ductal epithelium; 2 focal/connective tissue | 1 | 2 hetero/ductal epithelium |
| ND | 1 | 0/ductal epithelium | ||
| Bone marrow | 1 | 0/most cells; 3 scattered/cells | 1 | 0 |
| Diaphragm | ND | 1 | 0 | |
| Placenta | ND | 1 | 3 focal/cells lining trophoblasts | |
| ND | 1 | 3 scattered/cells lining trophoblasts | ||
| Skeletal muscle | ND | 1 | 0 | |
| ND | 1 | 2 hetero | ||
| Skin | 2 | 0/dermis; 3 homo/epidermis | 2 | 0/dermis; 3 hetero/epidermis, appendages |
| Spleen | 2 | 0/lymphoid; 3 scattered/cells, macrophages | 2 | 0/lymphoid |
| Thymus | 1 | 2 hetero/Hassall corpuscle; 3 focal/possibly medulla | 1 | 0/lymphoid; 3 scattered/plasma cells |
| ND | 1 | 0/lymphoid | ||
| Thyroid | ND | 1 | 0/follicle epithelium | |
| ND | 1 | 3 scattered/plasma cells | ||
| Tonsil | ND | 2 | 0/lymphoid; 3 hetero/squamous mucosa, scattered plasma cells | |
| Umbilical cord | 1 | 0/vessels are negative | ND | |
| OPM-2 cells | ||||
| Positive CNT | 1 | 3 homo | 1 | 3 homo |
| Namaiwa cells | ||||
| Negative CNT | 1 | 0 | 1 | 0 |
B-B4, or the control murine IgG1, was used at a concentration of 0.1 μg/mL and 2.5 μg/mL on frozen sections or on paraffin arrays respectively, to achieve equivalent staining in controls.
For staining intensity, 3 indicates strong; 2, moderate; 1, weak; and 0, no staining. Homo (homogeneous) indicates that more than 75% of cells were stained; hetero (heterogeneous), 25% to 75% of cells were stained; focal, less than 25% of cells were stained; and scattered, less than 5% of cells were stained.
ND indicates not done; CNT, control.
Corrected score with control antibody staining is subtracted from B-B4 staining