Lack of inhibitory effects of p100ΔN on the in vitro generation of Langerin (CD207)+ cells from CD34+ cord blood progenitors
Experiment . | p100ΔN . | Control . | RelB . | IκBα-SR . |
|---|---|---|---|---|
| 1 | 28* | 23 | 24 | 17 |
| 2 | 33 | 26 | 26 | 12 |
| 3 | 28 | 23 | 21 | NT |
| 4 | 37 | 25 | 27 | NT |
| 5 | 27 | 21 | 21 | NT |
| 6 | 32 | 27 | 25 | NT |
| 7 | 40 | 43 | 31 | 13 |
| 8 | 40 | 28 | 19 | NT |
| 9 | 18 | 14 | 20 | 9 |
| Mean | 31† | 26† | 24 | ND |
| SD | 7 | 8 | 4 | ND |
Experiment . | p100ΔN . | Control . | RelB . | IκBα-SR . |
|---|---|---|---|---|
| 1 | 28* | 23 | 24 | 17 |
| 2 | 33 | 26 | 26 | 12 |
| 3 | 28 | 23 | 21 | NT |
| 4 | 37 | 25 | 27 | NT |
| 5 | 27 | 21 | 21 | NT |
| 6 | 32 | 27 | 25 | NT |
| 7 | 40 | 43 | 31 | 13 |
| 8 | 40 | 28 | 19 | NT |
| 9 | 18 | 14 | 20 | 9 |
| Mean | 31† | 26† | 24 | ND |
| SD | 7 | 8 | 4 | ND |
In all experiments, CD34+ cells were cultured in DC generation cultures in the presence of cytokines and were gene transduced with retroviral vectors, as described in “Materials and methods.” Nine independent cord blood donor samples were analyzed.
NT indicates not tested; ND, not determined.
GFP+ cells were gated and analyzed for Langerin expression. Values represent percentages of Langerin- cells among GFP+ cells
Significantly higher percentages of Langerin+ cells among p100ΔN-transduced cells than among control-transduced cells. P = .0043; paired Student t test analysis