Oligonucleotide primers for PCR amplification and sequencing of the FGG exons and intron-exon junctions
Target . | Forward (5′ > 3′) . | Reverse (5′ > 3′) . |
|---|---|---|
| Exons 1-4 | FGG5′L: TCAGCTCCAGCCATTTGCAG | FGGi4R: TCAGGCATAATGTCACTGGG |
| Exons 5-6 | FGGx5L: TCAGGTCCACATTGTATTCC | FGGi6R: ATGAGCTACGGTTCACAAGG |
| Exon 7 | FGGx7L1: AGTTGATAGAACCAGTGCTC | FGGi7R: GACTCCTGGAGAAAATGGTG |
| Exon 8 | FGGx8L1: TTCCAAGGAAGCATCCTACG | FGGi8R: GTGGATTTCTTTAGAAGGGC |
| Exon 9-10 | FGGx9L: GTAACTGGCAATGCACTTCG | FGG3′R: GCTTTGCAAGTCCATTGTCC |
Target . | Forward (5′ > 3′) . | Reverse (5′ > 3′) . |
|---|---|---|
| Exons 1-4 | FGG5′L: TCAGCTCCAGCCATTTGCAG | FGGi4R: TCAGGCATAATGTCACTGGG |
| Exons 5-6 | FGGx5L: TCAGGTCCACATTGTATTCC | FGGi6R: ATGAGCTACGGTTCACAAGG |
| Exon 7 | FGGx7L1: AGTTGATAGAACCAGTGCTC | FGGi7R: GACTCCTGGAGAAAATGGTG |
| Exon 8 | FGGx8L1: TTCCAAGGAAGCATCCTACG | FGGi8R: GTGGATTTCTTTAGAAGGGC |
| Exon 9-10 | FGGx9L: GTAACTGGCAATGCACTTCG | FGG3′R: GCTTTGCAAGTCCATTGTCC |