Proportion and V3-23 gene mutation frequency of CD27+ B-cell subsets from blood and spleen of healthy individuals
. | . | IgD+CD27+ . | . | . | . | IgD-CD27+ . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | B cells, % . | . | Mutation frequency per 100 bp (total no. mutations; range of mutation per V sequence) . | . | B cells, % . | . | Mutation frequency per 100 bp (total no. mutations; range of mutation per V sequence) . | . | ||||||
| Donors . | Age . | Spleen . | Blood . | Spleen . | Blood . | Spleen . | Blood . | Spleen . | Blood . | ||||||
| C1 | 23 mo | 20 | 7.5 | 2.2 (58 mut; 0-17) | 3.1 (144 mut; 0-28) | 14 | 7 | 4.6 (225 mut; 0-37) | 4.7 (162 mut; 0-29) | ||||||
| C2 | 25 mo | 20 | 9 | 2.0 (82 mut; 0-12) | 3.8 (222 mut; 0-27) | 25 | 13 | 5.3 (216 mut; 8-29) | 4.9 (269 mut; 0-33) | ||||||
| C3 | 46 mo | 28 | 22 | 1.9 (95 mut; 0-25) | 2.8 (124 mut; 0-21) | 22 | 17 | 7.0 (182 mut; 6-31) | 6.6 (267 mut; 4-33) | ||||||
| C4 | 8 y | 31 | 35 | 2.4 (120 mut; 0-16) | 3.9 (183 mut; 2-26) | 10 | 16 | 4.8 (221 mut; 0-26) | 4.5 (90 mut; 8-20) | ||||||
| C5 | 9 y | 17 | 18 | 3.4 (109 mut; 1-17) | 5.2 (226 mut; 5-22) | 18 | 14 | 7.5 (196 mut; 6-32) | 5.2 (166 mut; 5-25) | ||||||
| 2.4 ± 0.6* | 3.8 ± 1* | 5.7 ± 1* | 5.2 ± 0.8* | ||||||||||||
. | . | IgD+CD27+ . | . | . | . | IgD-CD27+ . | . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| . | . | B cells, % . | . | Mutation frequency per 100 bp (total no. mutations; range of mutation per V sequence) . | . | B cells, % . | . | Mutation frequency per 100 bp (total no. mutations; range of mutation per V sequence) . | . | ||||||
| Donors . | Age . | Spleen . | Blood . | Spleen . | Blood . | Spleen . | Blood . | Spleen . | Blood . | ||||||
| C1 | 23 mo | 20 | 7.5 | 2.2 (58 mut; 0-17) | 3.1 (144 mut; 0-28) | 14 | 7 | 4.6 (225 mut; 0-37) | 4.7 (162 mut; 0-29) | ||||||
| C2 | 25 mo | 20 | 9 | 2.0 (82 mut; 0-12) | 3.8 (222 mut; 0-27) | 25 | 13 | 5.3 (216 mut; 8-29) | 4.9 (269 mut; 0-33) | ||||||
| C3 | 46 mo | 28 | 22 | 1.9 (95 mut; 0-25) | 2.8 (124 mut; 0-21) | 22 | 17 | 7.0 (182 mut; 6-31) | 6.6 (267 mut; 4-33) | ||||||
| C4 | 8 y | 31 | 35 | 2.4 (120 mut; 0-16) | 3.9 (183 mut; 2-26) | 10 | 16 | 4.8 (221 mut; 0-26) | 4.5 (90 mut; 8-20) | ||||||
| C5 | 9 y | 17 | 18 | 3.4 (109 mut; 1-17) | 5.2 (226 mut; 5-22) | 18 | 14 | 7.5 (196 mut; 6-32) | 5.2 (166 mut; 5-25) | ||||||
| 2.4 ± 0.6* | 3.8 ± 1* | 5.7 ± 1* | 5.2 ± 0.8* | ||||||||||||
Mut indicates mutation.
Average mutation frequency. Mutation frequencies were significantly higher in IgD- compared with IgD+ cells, both in the overall sample (P < 10-9) and after stratification by tissue type (blood, P = .03; spleen, P < 10-9; nonparametric Wilcoxon score test)