BCL2 primers and probe sequences used in quantitative RT-PCR
Primers* . | Sequence (5′-3′) . | Length of the product . |
|---|---|---|
| F1 | TTTTAGGAGACCGAAGTCCG | 392 bp (BCL2, exon 3) StaRT-PCR |
| R1 | AGCCAACGTGCCATGTGCTA | |
| F2 | TTGCTTTACGTGGCCTGTTTC | 94 bp (BCL2, exon 3) Real-time PCR |
| R2 | GAAGACCCTGAAGGACAGCCAT | |
| BCL2 probe | FAM–CCCACCCAGAGCCCTCCTGCC–TAMRA |
Primers* . | Sequence (5′-3′) . | Length of the product . |
|---|---|---|
| F1 | TTTTAGGAGACCGAAGTCCG | 392 bp (BCL2, exon 3) StaRT-PCR |
| R1 | AGCCAACGTGCCATGTGCTA | |
| F2 | TTGCTTTACGTGGCCTGTTTC | 94 bp (BCL2, exon 3) Real-time PCR |
| R2 | GAAGACCCTGAAGGACAGCCAT | |
| BCL2 probe | FAM–CCCACCCAGAGCCCTCCTGCC–TAMRA |
F indicates forward; and R, reverse.
F1 and R1 were applied in StaRT-PCR and F2 and R2 were used in real-time PCR