Sequences and Accession number of primers used to amplify mouse mRNA by RT-PCR
Gene . | Oligonucleotide sequences . | GenBank Accession no. . |
|---|---|---|
| Ndrg1 | 5′-TGCTTGCTCATTAGGTGTGTGATAGC-3′ FWD | NM_010884 |
| 5′-CCATCCTGAGATCTTAGAGGCAGC-3′ REV | ||
| HIF-1α | 5′-CTGGATGCCGGTGGTCTAGACAGT-3′ FWD | NM_010431 |
| 5′-CGAGAAGAAAAAGATGAGTTCTGAACGTCG-3′ REV | ||
| VEGF-1 | 5′-CCATGCCAAGTGGTCCCAG-3′ FWD | M95200.1 |
| 5′-GTCTTTCTTTGGTCTGCATTCACAT-3′ REV | ||
| β-actin | 5′-CTTCCTTCTTGGGTATGGAATCCTGT-3′ FWD | NM_00739 |
| 5′-CTCAGGAGGAGCAATGATCTTGATCTTC-3′ REV |
Gene . | Oligonucleotide sequences . | GenBank Accession no. . |
|---|---|---|
| Ndrg1 | 5′-TGCTTGCTCATTAGGTGTGTGATAGC-3′ FWD | NM_010884 |
| 5′-CCATCCTGAGATCTTAGAGGCAGC-3′ REV | ||
| HIF-1α | 5′-CTGGATGCCGGTGGTCTAGACAGT-3′ FWD | NM_010431 |
| 5′-CGAGAAGAAAAAGATGAGTTCTGAACGTCG-3′ REV | ||
| VEGF-1 | 5′-CCATGCCAAGTGGTCCCAG-3′ FWD | M95200.1 |
| 5′-GTCTTTCTTTGGTCTGCATTCACAT-3′ REV | ||
| β-actin | 5′-CTTCCTTCTTGGGTATGGAATCCTGT-3′ FWD | NM_00739 |
| 5′-CTCAGGAGGAGCAATGATCTTGATCTTC-3′ REV |
FWD indicates forward; REV, reverse.