Sequences and Accession number of primers used to amplify human transcripts by RT-PCR
Gene . | Oligonucleotide sequences . | GenBank Accession no. . |
|---|---|---|
| Ndrg1 | 5′-CCCTCGCGTTAGGCAGGTGA-3′ FWD | NM_006096 |
| 5′-AGGGGTACATGTACCCTGCG-3′ REV | ||
| TfR1 | 5′-TCAGGTCAAAGACAGCGCTCAAAACTC-3′ FWD | X01060 |
| 5′-AGTCTCCTTCCATATTCCCAAACAGCTTTT-3′ REV | ||
| VEGF-1 | 5′-AGTGCTCCAGCGCGCGCGCTCCC-3′ FWD | AF022375 |
| 5′-CTCTATCTTTCTTTGGTCTGCATTCACATT-3′ REV | ||
| WAF1 | 5′-CTCAGAGGAGGCGCCATGTC-3′ FWD | U03106 |
| 5′-GAGTGGTAGAAATCTGTCATGCTGG-3′ REV | ||
| β-actin | 5′-CCCGCCGCCAGCTCACCATGG-3′ FWD | X00351 |
| 5′-AAGGTCTCAAACATGATCTGGGTC-3′ REV |
Gene . | Oligonucleotide sequences . | GenBank Accession no. . |
|---|---|---|
| Ndrg1 | 5′-CCCTCGCGTTAGGCAGGTGA-3′ FWD | NM_006096 |
| 5′-AGGGGTACATGTACCCTGCG-3′ REV | ||
| TfR1 | 5′-TCAGGTCAAAGACAGCGCTCAAAACTC-3′ FWD | X01060 |
| 5′-AGTCTCCTTCCATATTCCCAAACAGCTTTT-3′ REV | ||
| VEGF-1 | 5′-AGTGCTCCAGCGCGCGCGCTCCC-3′ FWD | AF022375 |
| 5′-CTCTATCTTTCTTTGGTCTGCATTCACATT-3′ REV | ||
| WAF1 | 5′-CTCAGAGGAGGCGCCATGTC-3′ FWD | U03106 |
| 5′-GAGTGGTAGAAATCTGTCATGCTGG-3′ REV | ||
| β-actin | 5′-CCCGCCGCCAGCTCACCATGG-3′ FWD | X00351 |
| 5′-AAGGTCTCAAACATGATCTGGGTC-3′ REV |
FWD indicates forward; REV, reverse.