Comparison of the hepatocytic potential of transplanted cells from different sources
. | CD34+Lin- . | . | . | ||
|---|---|---|---|---|---|
| Time after transplantation . | CB . | Adult BM . | Mobilized PB . | ||
| 8 wk | |||||
| Human CD45, % | |||||
| Sheep 1 | 3.0 | 1.9 | 3.0 | ||
| Sheep 2 | 4.0 | 3.3 | 6.0 | ||
| Human hepatocytes, % | |||||
| Sheep 1 | 0.18 ± 0.01 | 0.6 ± 0.1 | 0.05 ± 0.01 | ||
| Sheep 2 | 0.22 ± 0.02 | 1.2 ± 0.05 | 0.08 ± 0.01 | ||
| 10 wk | |||||
| Human CD45, % | |||||
| Sheep 1 | 2.0 | 2.2 | 1.46 | ||
| Sheep 2 | 3.2 | — | 2.7 | ||
| Human hepatocytes, % | |||||
| Sheep 1 | 1.0 ± 0.01 | 3.0 ± 0.04 | 0.1 ± 0.0 | ||
| Sheep 2 | 2.0 ± 0.01 | — | 1.0 ± 0.01 | ||
| Human albumin, μg/mL | |||||
| Sheep 1 | 0.18 | 0.6 | 0.0 | ||
| Sheep 2 | 0.2 | 1.2 | 0.01 | ||
. | CD34+Lin- . | . | . | ||
|---|---|---|---|---|---|
| Time after transplantation . | CB . | Adult BM . | Mobilized PB . | ||
| 8 wk | |||||
| Human CD45, % | |||||
| Sheep 1 | 3.0 | 1.9 | 3.0 | ||
| Sheep 2 | 4.0 | 3.3 | 6.0 | ||
| Human hepatocytes, % | |||||
| Sheep 1 | 0.18 ± 0.01 | 0.6 ± 0.1 | 0.05 ± 0.01 | ||
| Sheep 2 | 0.22 ± 0.02 | 1.2 ± 0.05 | 0.08 ± 0.01 | ||
| 10 wk | |||||
| Human CD45, % | |||||
| Sheep 1 | 2.0 | 2.2 | 1.46 | ||
| Sheep 2 | 3.2 | — | 2.7 | ||
| Human hepatocytes, % | |||||
| Sheep 1 | 1.0 ± 0.01 | 3.0 ± 0.04 | 0.1 ± 0.0 | ||
| Sheep 2 | 2.0 ± 0.01 | — | 1.0 ± 0.01 | ||
| Human albumin, μg/mL | |||||
| Sheep 1 | 0.18 | 0.6 | 0.0 | ||
| Sheep 2 | 0.2 | 1.2 | 0.01 | ||
Data represent donor (human) hematopoietic activity and hepatocytic production in primary sheep recipients 8 and 10 weeks after transplantation. For each time point, 2 fetal sheep underwent transplantation with 2 × 104 CD34+Lin- cells from each of these sources.
Human hepatocyte values represent ranges of percentages found in 4 different sections from 4 different lobes of livers of the 2 animals per group to undergo transplantation. — indicates not done.