Table 2. Donor hepatocyte activity... | American Society of Hematology

Table 2.

Donor hepatocyte activity correlates with cell dose, donor hematopoietic engraftment, and time after transplantation

Cells injected	No. animals	Time after transplantation	Human hepatocytes, %	Human CD45⁺ cells, %
BM 34⁺Lin^-	2	3 wk	1.8 ± 0.05	0.8
			3 ± 0.1	1.1
BM 34⁺Lin^-	2	5 wk	2 ± 0.03	0.62
			3.2 ± 0.01	1.5
BM 34⁺Lin^-	2	4 mo	5.5 ± 0.2	0.87
			10 ± 0.08	6.4

Cells injected	No. animals	Time after transplantation	Human hepatocytes, %	Human CD45⁺ cells, %
BM 34⁺Lin^-	2	3 wk	1.8 ± 0.05	0.8
			3 ± 0.1	1.1
BM 34⁺Lin^-	2	5 wk	2 ± 0.03	0.62
			3.2 ± 0.01	1.5
BM 34⁺Lin^-	2	4 mo	5.5 ± 0.2	0.87
			10 ± 0.08	6.4

Values for human hepatocytes represent the average of hepatocytes found in 6 different liver sections from each of the animals analyzed at a given time point ± SD.

As it can be seen at each time point, a correlation exists between the percentage of human hepatocytes and the percentage of human CD45⁺ cells. Correlation coefficient was calculated according to the formula:

\(\mathrm{Cov}(\mathrm{X},\mathrm{Y})=\frac{1}{\mathrm{n}}{\sum_{\mathrm{i}=1}^{\mathrm{n}}}(\mathrm{x}_{\mathrm{i}}-{\mu}_{\mathrm{x}})(\mathrm{y}_{\mathrm{i}}-{\mu}_{\mathrm{y}})\)

Levels of CD45⁺ cells of hematopoietic engraftment reflect only the levels of human CD45⁺ cells in PB and BM.

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