Human HSCs generate significant numbers of hepatocytes in a noninjury fetal model
Phenotype . | Source . | Cell dose . | No. animals . | Time after transplantation . | Human hepatocytes, % . |
|---|---|---|---|---|---|
| CD34+Lin- | BM | 5.0 × 106 | 2 | 3 wk | 1.8 ± 0.1 |
| 3.0 ± 0.05 | |||||
| CD34+Lin- | BM | 1.3 × 105 | 2 | 5 wk | 2.0 ± 0.08 |
| 3.2 ± 0.1 | |||||
| CD34+Lin- | BM | 6.7 × 104 | 2 | 4 mo | 5.5 ± 0.2 |
| 10 ± 0.8 | |||||
| CD34+Lin- | CB | 3.8 × 105 | 2 | 2 mo | 1.0 ± 0.01 |
| 2.0 ± 0.05 | |||||
| CD34-Lin- | CB | 1.1 × 106 | 1 | 2 mo | 2.0 ± 1.0 |
| CD34-Lin- | CB | 5.0 × 105 | 1 | 11 mo | 17.0 ± 2.0 |
Phenotype . | Source . | Cell dose . | No. animals . | Time after transplantation . | Human hepatocytes, % . |
|---|---|---|---|---|---|
| CD34+Lin- | BM | 5.0 × 106 | 2 | 3 wk | 1.8 ± 0.1 |
| 3.0 ± 0.05 | |||||
| CD34+Lin- | BM | 1.3 × 105 | 2 | 5 wk | 2.0 ± 0.08 |
| 3.2 ± 0.1 | |||||
| CD34+Lin- | BM | 6.7 × 104 | 2 | 4 mo | 5.5 ± 0.2 |
| 10 ± 0.8 | |||||
| CD34+Lin- | CB | 3.8 × 105 | 2 | 2 mo | 1.0 ± 0.01 |
| 2.0 ± 0.05 | |||||
| CD34-Lin- | CB | 1.1 × 106 | 1 | 2 mo | 2.0 ± 1.0 |
| CD34-Lin- | CB | 5.0 × 105 | 1 | 11 mo | 17.0 ± 2.0 |
Human HSCs were isolated by cell sorting and were transplanted intraperitoneally into preimmune sheep fetuses at 55 to 65 days of gestation. Values for human hepatocytes represent the average ± SD of hepatocytes found in each of the animals analyzed at a given time point.