Table 1. Experimental assessment of... | American Society of Hematology

Table 1.

Experimental assessment of disinfection procedures

Procedures	Spore suspension, log₁₀ spores/mL	Inoculated Petri dishes, log₁₀ spores/mL
Controls	5.75 ± 0.49	5.80 ± 0.75
Freezing at -20°C, 24 h	5.15 ± 0.34	5.70 ± 0.63
MW irradiation
800 W, 1 min	3.45 ± 1.40	5.70 ± 0.82
800 W, 2 min	0*	5.40 ± 0.70
Oven heating
150°C, 5 min	0.05 ± 0.16*	2.75 ± 0.72*
150°C, 10 min	0*	1.55 ± 0.50*
150°C, 15 min	0*	2.50 ± 0.91*
220°C, 5 min	0.10 ± 0.32*	0.45 ± 0.76*
220°C, 10 min	0*	0.10 ± 0.32*
220°C, 15 min	0*	0*
Hot water
60°C	5.75 ± 0.54	5.60 ± 0.57
100°C	5.15 ± 0.63	4.95 ± 0.76
70% ethanol	—	0*

Procedures	Spore suspension, log₁₀ spores/mL	Inoculated Petri dishes, log₁₀ spores/mL
Controls	5.75 ± 0.49	5.80 ± 0.75
Freezing at -20°C, 24 h	5.15 ± 0.34	5.70 ± 0.63
MW irradiation
800 W, 1 min	3.45 ± 1.40	5.70 ± 0.82
800 W, 2 min	0*	5.40 ± 0.70
Oven heating
150°C, 5 min	0.05 ± 0.16*	2.75 ± 0.72*
150°C, 10 min	0*	1.55 ± 0.50*
150°C, 15 min	0*	2.50 ± 0.91*
220°C, 5 min	0.10 ± 0.32*	0.45 ± 0.76*
220°C, 10 min	0*	0.10 ± 0.32*
220°C, 15 min	0*	0*
Hot water
60°C	5.75 ± 0.54	5.60 ± 0.57
100°C	5.15 ± 0.63	4.95 ± 0.76
70% ethanol	—	0*

Results are shown for 10-mL suspensions containing 3 × 10⁸ spores of A fumigatus/mL and Petri dishes contaminated with 3 × 10⁸ spores of A fumigatus (mean log value ± SD of 10 determinations).

— indicates not done.

Significantly different from the untreated control group at P < .05.

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