IC50 values in neoplastic and normal cells
. | Neoplastic cells . | . | . | Normal cells . | ||
|---|---|---|---|---|---|---|
. | SK-N-MC neuroepithelioma . | SK-Mel-28 melanoma . | MCF-7 breast cancer . | MRC-5 fibroblasts . | ||
| DFO | 5 ± 2 | 15 ± 7 | 14 ± 9 | > 25 | ||
| 311 | 0.3 ± 0.2 | 0.9 ± 0.5 | — | > 25 | ||
| DpT | > 25 | > 25 | > 25 | > 25 | ||
| Dp2mT | > 25 | > 25 | > 25 | > 25 | ||
| Dp4mT | 0.19 ± 0.1 | 0.6 ± 0.5 | 0.3 ± 0.2 | > 25 | ||
| Dp44mT | 0.03 ± 0.01 | 0.06 ± 0.03 | 0.06 ± 0.01 | > 25 | ||
| Dp4eT | 0.06 ± 0.01 | 0.09 ± 0.06 | 0.08 ± 0.01 | > 25 | ||
| Dp4aT | 0.06 ± 0.01 | 0.10 ± 0.06 | 0.07 ± 0.01 | > 25 | ||
| Dp4pT | 0.05 ± 0.006 | 0.09 ± 0.05 | 0.07 ± 0.01 | > 25 | ||
| 3-AP | 0.26 ± 0.01 | 2.6 ± 0.6 | 3.0 ± 1.5 | — | ||
| Doxorubicin | 0.02 ± 0.01 | 0.35 ± 0.09 | 0.6 ± 0.2 | — | ||
. | Neoplastic cells . | . | . | Normal cells . | ||
|---|---|---|---|---|---|---|
. | SK-N-MC neuroepithelioma . | SK-Mel-28 melanoma . | MCF-7 breast cancer . | MRC-5 fibroblasts . | ||
| DFO | 5 ± 2 | 15 ± 7 | 14 ± 9 | > 25 | ||
| 311 | 0.3 ± 0.2 | 0.9 ± 0.5 | — | > 25 | ||
| DpT | > 25 | > 25 | > 25 | > 25 | ||
| Dp2mT | > 25 | > 25 | > 25 | > 25 | ||
| Dp4mT | 0.19 ± 0.1 | 0.6 ± 0.5 | 0.3 ± 0.2 | > 25 | ||
| Dp44mT | 0.03 ± 0.01 | 0.06 ± 0.03 | 0.06 ± 0.01 | > 25 | ||
| Dp4eT | 0.06 ± 0.01 | 0.09 ± 0.06 | 0.08 ± 0.01 | > 25 | ||
| Dp4aT | 0.06 ± 0.01 | 0.10 ± 0.06 | 0.07 ± 0.01 | > 25 | ||
| Dp4pT | 0.05 ± 0.006 | 0.09 ± 0.05 | 0.07 ± 0.01 | > 25 | ||
| 3-AP | 0.26 ± 0.01 | 2.6 ± 0.6 | 3.0 ± 1.5 | — | ||
| Doxorubicin | 0.02 ± 0.01 | 0.35 ± 0.09 | 0.6 ± 0.2 | — | ||
Each IC50 value (given in μM) is the mean ± SEM of at least 3 experiments performed. Cells were incubated in the presence and absence of DFO, 311, 3-aminopyridine-2-carboxaldehyde thiosemicarbazone, doxorubicin, and the DpT series of chelators (0-25 μM) for 72 hours at 37°C. After this incubation period, cellular proliferation was determined by the MTT assay.23 — indicates not determined.