Table 1.

Proteins that are differentially expressed at 8, 48, 72, and 96 hours of ATRA treatment through 2-DE

Accession no.
Peptides sequenced
Protein expression†
mRNA expression‡
Spot
Protein name
Functional classification
Mr, kDa*
pl*
8 h
48 h
72 h
96 h
D1   Q04637   eIF4G   Protein synthesis   153   5.18   4   0.71   0.50   0.21   0.17   Unchanged§ 
D2  AAH19816  70-kDa hsp 8   Chaperone   70.9   5.37   15   0.90   0.25   0.34∥  0.39   Unknown  
D3   P04765   eIF4A-1   Protein synthesis   46.1   5.32   6   0.95   0.46   0.34   0.40   Unchanged§ 
D4   Q9Y230   48-kDa TATA   Transcription   54.1   5.49   2   0.70   0.49   0.43   0.45   Unknown  
D5   P06753-01   Tropomyosin α3   Cellular organization   29.0   4.75   3   1.1∥  0.61   0.50   0.50∥  Unknown  
D6   P42655   14-3-3ϵ   Signal transduction   29.1   4.63   3   0.83   0.56   0.35   0.41   Unknown  
D7   P56537   eIF 6   Protein synthesis   26.5   4.56   2   0.90   0.60   0.34   0.38   Unknown  
D8   P52597   hnRNP F   mRNA splicing   45.6   5.38   2   0.76   0.49   0.40   0.38   Unchanged§ 
U1   Q14974   Importin β-1   Cellular transport   97.2   4.68   5   1.0   1.8∥  2.0   2.4   Repressed24  
U2   P50213   NAD   Metabolism   39.5   6.47   2   ND   2.0   3.8   4.3   Unknown  
U3   P47755   CapZ α-2   Structural   33.0   5.57   2   1.0   2.1   2.7   3.7   Unknown  
U4   Q9UL46   PA28 β   Cellular defense   27.3   5.44   4   1.2   2.1   1.8   2.3   Induced24  
U5   P47756   CapZ β   Structural   30.6   5.69   4   0.90∥  1.6   2.1   3.8   Unknown  
U6   P78417   GSTO 1-1   Not defined   27.5   6.23   7   0.80   1.7   1.9   1.7   Unknown  
U7   P18669   PGAM-B   Glycolysis   28.6   6.75   7   1.6   6.5∥  22   20   Induced25  
U8   Q14019   Coactosin-like   Cellular organization   15.9   5.54   2   1.1∥  2.2   3.3   2.5   Unknown  
U9   P29373   CRABP-II   Retinoid metabolism   15.6   5.43   2   1.5   2.7   3.7   4.2   Unknown  
U10   P27482   CLP   Signal transduction   16.7   4.30   2   1.1∥  1.5   1.9∥  1.7   Induced23  
U11   P05387   60S P2 protein   Protein synthesis   11.7   4.42   4   ND   2.5   3.9   4.7   Unknown  
U12   P19105   Myosin RLC   Cellular organization   19.7   4.67   2   1.8   3.0   4.1   4.4   Unknown  
U13   P52566   Rho GDI 2   Signal transduction   22.9   5.10   4   1.3   2.0   2.6   2.6   Unknown  
U14
 		 P29218
 		 IMPase
 		 Signal transduction
 		 30.2
 		 5.16
 		 2
 		 ND
 		 2.1∥
 		 2.9
 		 2.5
 		 Unknown
 
Accession no.
Peptides sequenced
Protein expression†
mRNA expression‡
Spot
Protein name
Functional classification
Mr, kDa*
pl*
8 h
48 h
72 h
96 h
D1   Q04637   eIF4G   Protein synthesis   153   5.18   4   0.71   0.50   0.21   0.17   Unchanged§ 
D2  AAH19816  70-kDa hsp 8   Chaperone   70.9   5.37   15   0.90   0.25   0.34∥  0.39   Unknown  
D3   P04765   eIF4A-1   Protein synthesis   46.1   5.32   6   0.95   0.46   0.34   0.40   Unchanged§ 
D4   Q9Y230   48-kDa TATA   Transcription   54.1   5.49   2   0.70   0.49   0.43   0.45   Unknown  
D5   P06753-01   Tropomyosin α3   Cellular organization   29.0   4.75   3   1.1∥  0.61   0.50   0.50∥  Unknown  
D6   P42655   14-3-3ϵ   Signal transduction   29.1   4.63   3   0.83   0.56   0.35   0.41   Unknown  
D7   P56537   eIF 6   Protein synthesis   26.5   4.56   2   0.90   0.60   0.34   0.38   Unknown  
D8   P52597   hnRNP F   mRNA splicing   45.6   5.38   2   0.76   0.49   0.40   0.38   Unchanged§ 
U1   Q14974   Importin β-1   Cellular transport   97.2   4.68   5   1.0   1.8∥  2.0   2.4   Repressed24  
U2   P50213   NAD   Metabolism   39.5   6.47   2   ND   2.0   3.8   4.3   Unknown  
U3   P47755   CapZ α-2   Structural   33.0   5.57   2   1.0   2.1   2.7   3.7   Unknown  
U4   Q9UL46   PA28 β   Cellular defense   27.3   5.44   4   1.2   2.1   1.8   2.3   Induced24  
U5   P47756   CapZ β   Structural   30.6   5.69   4   0.90∥  1.6   2.1   3.8   Unknown  
U6   P78417   GSTO 1-1   Not defined   27.5   6.23   7   0.80   1.7   1.9   1.7   Unknown  
U7   P18669   PGAM-B   Glycolysis   28.6   6.75   7   1.6   6.5∥  22   20   Induced25  
U8   Q14019   Coactosin-like   Cellular organization   15.9   5.54   2   1.1∥  2.2   3.3   2.5   Unknown  
U9   P29373   CRABP-II   Retinoid metabolism   15.6   5.43   2   1.5   2.7   3.7   4.2   Unknown  
U10   P27482   CLP   Signal transduction   16.7   4.30   2   1.1∥  1.5   1.9∥  1.7   Induced23  
U11   P05387   60S P2 protein   Protein synthesis   11.7   4.42   4   ND   2.5   3.9   4.7   Unknown  
U12   P19105   Myosin RLC   Cellular organization   19.7   4.67   2   1.8   3.0   4.1   4.4   Unknown  
U13   P52566   Rho GDI 2   Signal transduction   22.9   5.10   4   1.3   2.0   2.6   2.6   Unknown  
U14
 		 P29218
 		 IMPase
 		 Signal transduction
 		 30.2
 		 5.16
 		 2
 		 ND
 		 2.1∥
 		 2.9
 		 2.5
 		 Unknown
 

Mr indicates molecular weight; D, down-regulation; U, up-regulation; eIF, eukaryotic translation initiation factor; hsp, heat shock protein; NAD, isocitrate dehydrogenase; ND, no data were found; CapZ, F-actin capping protein; PA28 β, proteasome activator complex subunit 2; GSTO 1-1, glutathione transferase omega 1; PGAM-B, phosphoglycerate mutase 1; CRABP-II, retinoic acid-binding protein II; CLP, calmodulin-related protein NB-1; Rho GDI 2, Rho guanosine diphosphate (GDP)-dissociation inhibitor 2; IMPase, inositol-1(or 4)-monophosphatase.

*

Theoretical.

†

ATRA-treated divided by nontreated. All values are statistically significant; P < .05 unless otherwise indicated.

‡

The mRNA expression is as displayed in references Liu et al23  (for 0 to 72 hours), Yang et al24  (for 0 to 96 hours), or Lee et al25  (for 0 to 48 hours) or by personal communication. Unchanged mRNA expression ratios (for ATRA treated divided by nontreated) were within 0.8 to 1.2 as a function of various ATRA treatment time points.

∥

P > .05.

§

See Figure 4.

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