Sequence and annealing temperature of RT-PCR primers used
Name . | Forward primer . | Reverse primer . | Temperature, °C . |
|---|---|---|---|
| β-actin | 5′ ATGAAGATCCTGACCGAGCG 3′ | 5′ TACTTGCGCTCAGGAGGAGC 3′ | 58 |
| Rex-1 | 5′ CGTGTAACATACACCATCCG 3′ | 5′ GAAATCCTCTTCCAGAATGG 3′ | 55 |
| Gata-1 | 5′ TAAGGTGGCTGAATCCTCTGCATC 3′ | 5′ ACGTTCTTGGACACCTTGAAGACGG 3′ | 64 |
| SCL | 5′ ATTGCACACACGGGATTCTG 3′ | 5′ GAATTCAGGGTCTTCCTTAG 3′ | 50 |
| EKLF | 5′ ACCACCCTGGGACAGTTTCT 3′ | 5′ GAAGGGTCCTCCGATTTCAG 3′ | 60 |
| Epo-R | 5′ TTCTCCTCGCTATCACCGCATC 3′ | 5′ CCTCAAACTCGCTCTCTGGGCT 3′ | 64 |
| α-globin | 5′ CTCTCTGGGGAAGACAAAAGCAAC 3′ | 5′ GGTGGCTAGCCAAGGTCACCAGCA 3′ | 55 |
| βmajor-globin | 5′ CACAAACCCCAGAAACAGACA 3′ | 5′ CTGACAGATGCTCTCTTGGG 3′ | 55 |
| βH1-globin | 5′ CTCAAGGAGACCTTTGCTCA 3′ | 5′ AGTCCCCATGGAGTCAAAGA 3′ | 55 |
| ϵ-globin | 5′ GGAGAGTCCATTAAGAACCTAGACAA 3′ | 5′ CTGTGAATTCATTGCCGAAGTAC 3′ | 55 |
| ζ-globin | 5′ GCTCAGGCCGAGCCCATTGG 3′ | 5′ TAGCGGTACTTCTCAGTCAG 3′ | 55 |
| CEBPα | 5′ GGTGGACAAGAACAGCAACGAG 3′ | 5′ TAGAGATCCAGCGACCCGAAAC 3′ | 55 |
| CEBPϵ | 5′ AGCCCCCGACACCCTTGATGA 3′ | 5′ GTCCCCTTCTCAAGGCACCCT 3′ | 58 |
| G-CSFR | 5′ CCCCTCAAACCTATCCTGCCTC 3′ | 5′ TCCAGGCAGAGATGAGCGAATG 3′ | 58 |
| GM-CSF | 5′ TGAGGAGGATGTGGCTGCAGAA 3′ | 5′ TGTGCCACATCTCTTGGTCCCT 3′ | 65 |
| MPO | 5′ ATGCAGTGGGGACAGTTTCTG 3′ | 5′ GTCGTTGTAGGATCGGTACTG 3′ | 55 |
| c-fms | 5′ GCGATGTGTGAGCAATGGCAGT 3′ | 5′ AGACCGTTTTGCGTAAGACCTG 3′ | 60 |
| ICSBP | 5′ GTCCCAACTGGACATTTCCG 3′ | 5′ CATTCACGCAGCCAGCAG 3′ | 58 |
| IL-7Rα | 5′ TTACTTCAAAGGCTTCTGGAG 3′ | 5′ CTGGCTTCAACGCCTTTCACCTCA 3′ | 58 |
| Pax5 | 5′ CGGGTCAGCCATGGTTGTG 3′ | 5′ GTGCTGTCTCTCAAACACG 3′ | 52 |
| Gata-3 | 5′ TATGTGCCCGAGTACAGCTC 3′ | 5′ TGTAGTACAGCCCACAGGCA 3′ | 57 |
| Ets-1 | 5′ CTACGGTATCGAGCATGCTCAGTG 3′ | 5′ AAGGTGTCTGTCTGGAGAGGGTCC 3′ | 58 |
| Flt3 | 5′ TCTTGAGACCGTTACAAACC 3′ | 5′ ATGTCTGTTCCGAACAACTC 3′ | 54 |
| CXCR4 | 5′ AAATTTTTGTGTAAGGCTGTC 3′ | 5′ CCCAAAAGGATGAAGGAGTCG 3′ | 52 |
Name . | Forward primer . | Reverse primer . | Temperature, °C . |
|---|---|---|---|
| β-actin | 5′ ATGAAGATCCTGACCGAGCG 3′ | 5′ TACTTGCGCTCAGGAGGAGC 3′ | 58 |
| Rex-1 | 5′ CGTGTAACATACACCATCCG 3′ | 5′ GAAATCCTCTTCCAGAATGG 3′ | 55 |
| Gata-1 | 5′ TAAGGTGGCTGAATCCTCTGCATC 3′ | 5′ ACGTTCTTGGACACCTTGAAGACGG 3′ | 64 |
| SCL | 5′ ATTGCACACACGGGATTCTG 3′ | 5′ GAATTCAGGGTCTTCCTTAG 3′ | 50 |
| EKLF | 5′ ACCACCCTGGGACAGTTTCT 3′ | 5′ GAAGGGTCCTCCGATTTCAG 3′ | 60 |
| Epo-R | 5′ TTCTCCTCGCTATCACCGCATC 3′ | 5′ CCTCAAACTCGCTCTCTGGGCT 3′ | 64 |
| α-globin | 5′ CTCTCTGGGGAAGACAAAAGCAAC 3′ | 5′ GGTGGCTAGCCAAGGTCACCAGCA 3′ | 55 |
| βmajor-globin | 5′ CACAAACCCCAGAAACAGACA 3′ | 5′ CTGACAGATGCTCTCTTGGG 3′ | 55 |
| βH1-globin | 5′ CTCAAGGAGACCTTTGCTCA 3′ | 5′ AGTCCCCATGGAGTCAAAGA 3′ | 55 |
| ϵ-globin | 5′ GGAGAGTCCATTAAGAACCTAGACAA 3′ | 5′ CTGTGAATTCATTGCCGAAGTAC 3′ | 55 |
| ζ-globin | 5′ GCTCAGGCCGAGCCCATTGG 3′ | 5′ TAGCGGTACTTCTCAGTCAG 3′ | 55 |
| CEBPα | 5′ GGTGGACAAGAACAGCAACGAG 3′ | 5′ TAGAGATCCAGCGACCCGAAAC 3′ | 55 |
| CEBPϵ | 5′ AGCCCCCGACACCCTTGATGA 3′ | 5′ GTCCCCTTCTCAAGGCACCCT 3′ | 58 |
| G-CSFR | 5′ CCCCTCAAACCTATCCTGCCTC 3′ | 5′ TCCAGGCAGAGATGAGCGAATG 3′ | 58 |
| GM-CSF | 5′ TGAGGAGGATGTGGCTGCAGAA 3′ | 5′ TGTGCCACATCTCTTGGTCCCT 3′ | 65 |
| MPO | 5′ ATGCAGTGGGGACAGTTTCTG 3′ | 5′ GTCGTTGTAGGATCGGTACTG 3′ | 55 |
| c-fms | 5′ GCGATGTGTGAGCAATGGCAGT 3′ | 5′ AGACCGTTTTGCGTAAGACCTG 3′ | 60 |
| ICSBP | 5′ GTCCCAACTGGACATTTCCG 3′ | 5′ CATTCACGCAGCCAGCAG 3′ | 58 |
| IL-7Rα | 5′ TTACTTCAAAGGCTTCTGGAG 3′ | 5′ CTGGCTTCAACGCCTTTCACCTCA 3′ | 58 |
| Pax5 | 5′ CGGGTCAGCCATGGTTGTG 3′ | 5′ GTGCTGTCTCTCAAACACG 3′ | 52 |
| Gata-3 | 5′ TATGTGCCCGAGTACAGCTC 3′ | 5′ TGTAGTACAGCCCACAGGCA 3′ | 57 |
| Ets-1 | 5′ CTACGGTATCGAGCATGCTCAGTG 3′ | 5′ AAGGTGTCTGTCTGGAGAGGGTCC 3′ | 58 |
| Flt3 | 5′ TCTTGAGACCGTTACAAACC 3′ | 5′ ATGTCTGTTCCGAACAACTC 3′ | 54 |
| CXCR4 | 5′ AAATTTTTGTGTAAGGCTGTC 3′ | 5′ CCCAAAAGGATGAAGGAGTCG 3′ | 52 |