Cellular iron concentration (pg/cell) after labeling with different ferumoxides-protamine sulfate (FE-Pro) ratios and incubation times
FE-Pro complexes Cell types (incubation time) . | 0:0 μg/mL . | 50:0 μg/mL . | 50:1.5 μg/mL . | 50:2.25 μg/mL . | 50:3 μg/mL . | 50:4.5 μg/mL . | 50:9 μg/mL . |
|---|---|---|---|---|---|---|---|
| HSC (overnight) | 0.11 ± 0.10 | ND | ND | ND | 2.01 ± 0.10 | ND | ND |
| MSC (overnight) | 0.18 ± 0.10 | ND | ND | ND | 10.94 ± 1.86 | 9.75 ± 3.03 | ND |
| LADMAC (2 h) | 0.31 ± 0.13 | 0.67 ± 0.09* | ND | ND | 3.73 ± 0.26 | 6.02 ± 0.09 | 5.58 ± 0.09 |
| LADMAC (overnight) | 0.31 ± 0.13 | 2.81 ± 0.33* | ND | ND | 11.25 ± 1.13 | 13.58 ± 1.50 | 12.64 ± 1.40 |
| Mouse splenocyte (overnight) | 0.21 ± 0.09 | ND | 0.54 ± 0.05 | 0.85 ± 0.05 | 1.47 ± 0.03 | ND | ND |
| Mouse macrophage (overnight) | 0.05 ± 0.02 | ND | 17.90 ± 1.85 | ND | 17.31 ± 1.06 | ND | 17.89 ± 1.82 |
FE-Pro complexes Cell types (incubation time) . | 0:0 μg/mL . | 50:0 μg/mL . | 50:1.5 μg/mL . | 50:2.25 μg/mL . | 50:3 μg/mL . | 50:4.5 μg/mL . | 50:9 μg/mL . |
|---|---|---|---|---|---|---|---|
| HSC (overnight) | 0.11 ± 0.10 | ND | ND | ND | 2.01 ± 0.10 | ND | ND |
| MSC (overnight) | 0.18 ± 0.10 | ND | ND | ND | 10.94 ± 1.86 | 9.75 ± 3.03 | ND |
| LADMAC (2 h) | 0.31 ± 0.13 | 0.67 ± 0.09* | ND | ND | 3.73 ± 0.26 | 6.02 ± 0.09 | 5.58 ± 0.09 |
| LADMAC (overnight) | 0.31 ± 0.13 | 2.81 ± 0.33* | ND | ND | 11.25 ± 1.13 | 13.58 ± 1.50 | 12.64 ± 1.40 |
| Mouse splenocyte (overnight) | 0.21 ± 0.09 | ND | 0.54 ± 0.05 | 0.85 ± 0.05 | 1.47 ± 0.03 | ND | ND |
| Mouse macrophage (overnight) | 0.05 ± 0.02 | ND | 17.90 ± 1.85 | ND | 17.31 ± 1.06 | ND | 17.89 ± 1.82 |
Data are expressed as mean ± 1 SD from 3 to 4 samples.
MSC indicates mesenchymal stem cells. All other abbreviations are explained in Table 2.
Significant differences (P < .001) compared with cells incubated with Fe-Pro complexes at corresponding time points.