Proliferative activity of different cell lines incubated with different doses of protamine sulfate alone
Cell types . | 0 μg/mL . | 1.5 μg/mL . | 3 μg/mL . | 6 μg/mL . | 12.5 μg/mL . | 25 μg/mL . | 50 μg/mL . |
|---|---|---|---|---|---|---|---|
| HSC, 24 h | 100.00 ± 5.21 | 97.06 ± 3.98 | ND | 97.06 ± 7.72 | 103.31 ± 4.17 | 99.27 ± 3.30 | 100.74 ± 3.18 |
| Mouse splenocyte, 24 h | 99.95 ± 1.62 | 101.08 ± 4.07 | 96.76 ± 1.62 | 93.37 ± 3.49 | 93.96 ± 2.30 | 94.06 ± 5.35 | 94.39 ± 2.38 |
| Mouse splenocyte, 72 h | 100.09 ± 3.56 | 101.46 ± 1.65 | 101.74 ± 3.10 | 101.00 ± 1.53 | 100.82 ± 3.05 | 98.86 ± 1.28 | 93.65 ± 3.29 |
| LADMAC, 24 h | 99.96 ± 4.79 | 96.38 ± 3.54 | 98.01 ± 1.21 | 94.24 ± 3.02 | 96.25 ± 2.84 | 97.47 ± 1.73 | 94.70 ± 4.22 |
| LADMAC, 72 h | 99.98 ± 5.81 | 95.26 ± 1.89 | 94.98 ± 5.27 | 99.06 ± 2.67 | 94.40 ± 3.37 | 97.32 ± 2.80 | 94.38 ± 1.71 |
Cell types . | 0 μg/mL . | 1.5 μg/mL . | 3 μg/mL . | 6 μg/mL . | 12.5 μg/mL . | 25 μg/mL . | 50 μg/mL . |
|---|---|---|---|---|---|---|---|
| HSC, 24 h | 100.00 ± 5.21 | 97.06 ± 3.98 | ND | 97.06 ± 7.72 | 103.31 ± 4.17 | 99.27 ± 3.30 | 100.74 ± 3.18 |
| Mouse splenocyte, 24 h | 99.95 ± 1.62 | 101.08 ± 4.07 | 96.76 ± 1.62 | 93.37 ± 3.49 | 93.96 ± 2.30 | 94.06 ± 5.35 | 94.39 ± 2.38 |
| Mouse splenocyte, 72 h | 100.09 ± 3.56 | 101.46 ± 1.65 | 101.74 ± 3.10 | 101.00 ± 1.53 | 100.82 ± 3.05 | 98.86 ± 1.28 | 93.65 ± 3.29 |
| LADMAC, 24 h | 99.96 ± 4.79 | 96.38 ± 3.54 | 98.01 ± 1.21 | 94.24 ± 3.02 | 96.25 ± 2.84 | 97.47 ± 1.73 | 94.70 ± 4.22 |
| LADMAC, 72 h | 99.98 ± 5.81 | 95.26 ± 1.89 | 94.98 ± 5.27 | 99.06 ± 2.67 | 94.40 ± 3.37 | 97.32 ± 2.80 | 94.38 ± 1.71 |
Means expressed as percentage of control with standard deviations from 4 to 6 samples.
HSC indicates human CD34+ cell; ND, not done; and LADMAC, mouse lymphoblastic lymphoma cell line.