Cyclin D2–overexpressing LSK cells produce enhanced numbers of mature myeloid cells as well as immature progenitors
. | Fold expansion total cells (SD) . | % CD11b+Gr1+ (SD) . | % LSK (SD) . | Fold expansion LSK cells (SD) . |
|---|---|---|---|---|
| Control | 55 (47) | 23.9 (18.0) | 3.4 (1.5) | 1.9 (0.8) |
| Cyclin D2 | 272 (190) | 26.0 (5.5) | 4.7 (3.0) | 12.8 (8.2) |
. | Fold expansion total cells (SD) . | % CD11b+Gr1+ (SD) . | % LSK (SD) . | Fold expansion LSK cells (SD) . |
|---|---|---|---|---|
| Control | 55 (47) | 23.9 (18.0) | 3.4 (1.5) | 1.9 (0.8) |
| Cyclin D2 | 272 (190) | 26.0 (5.5) | 4.7 (3.0) | 12.8 (8.2) |
Control- and cyclin D2–transduced LSK cells were cultured in serum-free medium with SFM for 7 days. Cells were enumerated and analyzed for CD11b/Gr1 and LSK phenotypes by FACS. Data represent total numbers of GFP+ cells and are mean (SD) values from 3 experiments. Mean transduction efficiencies were 87% (70%-98%) and 46% (39%-60%) for control and cyclin D2 vectors, respectively.