Overexpression of cyclin D2 in hematopoietic progenitors accelerates erythro-myeloid reconstitution following transplantation of lethally ablated recipients
. | Day 7 . | . | Day 17 . | . | ||
|---|---|---|---|---|---|---|
. | Control . | Cyclin D2 . | Control . | Cyclin D2 . | ||
| WBC, × 109/L (SD) | 0.16 (0.07) | 0.22 (0.07) | 0.24 (0.13) | 0.50 (0.15)* | ||
| RBC, × 109/L (SD) | 5.78 (1.83) | 6.32 (1.56) | 3.37 (0.75) | 5.03 (1.32)* | ||
| PLT, × 109/L (SD) | 48.8 (16.5) | 80.5 (26.9)* | 188 (270) | 358 (390) | ||
| Spleen cellularity × 106 (SD) | 22.2 (9.8) | 50.6 (33.5) | 175 (23.1) | 259 (30.9)* | ||
| Spleen CFU-GM (SD) | 171 (81.6) | 1152 (1053)* | 7850 (3430) | 39 400 (15 900)* | ||
| BM cellularity/hind limbs × 106 (SD) | 7.1 (2.4) | 12.8 (5.4)* | 13.2 (5.1) | 23.3 (8.0)* | ||
| BM CFU-GM/hind limbs (SD) | 122 (85.0) | 276 (248) | 298 (375) | 1 590 (1610)* | ||
. | Day 7 . | . | Day 17 . | . | ||
|---|---|---|---|---|---|---|
. | Control . | Cyclin D2 . | Control . | Cyclin D2 . | ||
| WBC, × 109/L (SD) | 0.16 (0.07) | 0.22 (0.07) | 0.24 (0.13) | 0.50 (0.15)* | ||
| RBC, × 109/L (SD) | 5.78 (1.83) | 6.32 (1.56) | 3.37 (0.75) | 5.03 (1.32)* | ||
| PLT, × 109/L (SD) | 48.8 (16.5) | 80.5 (26.9)* | 188 (270) | 358 (390) | ||
| Spleen cellularity × 106 (SD) | 22.2 (9.8) | 50.6 (33.5) | 175 (23.1) | 259 (30.9)* | ||
| Spleen CFU-GM (SD) | 171 (81.6) | 1152 (1053)* | 7850 (3430) | 39 400 (15 900)* | ||
| BM cellularity/hind limbs × 106 (SD) | 7.1 (2.4) | 12.8 (5.4)* | 13.2 (5.1) | 23.3 (8.0)* | ||
| BM CFU-GM/hind limbs (SD) | 122 (85.0) | 276 (248) | 298 (375) | 1 590 (1610)* | ||
Five thousand LSK cells transduced with control- or cyclin D2–containing vectors were transplanted without any support cells into lethally ablated (975 cGy) adult recipients. A total of 6 mice at day 7, and 8 mice at day 14 in each group were analyzed in 2 separate experiments. Transduction efficiency was more than 90% for both control and cyclin D2 vectors. Data are shown as means (SD). WBC indicates white blood cells; RBC, red blood cells; and PLT, platelet.
P < .05.