Table 1.

CD80 and CD86 expression by BCP-ALL cells activated or not activated with CD40L



Positive cells, %
CD80
CD86
Patient
-CD40L
+CD40L
-CD40L
+CD40L
1   3   10   7   15  
2   0   0   0   10  
3   5   25   34   55  
4   0   0   80   90  
5   9   20   64   78  
6   0   20   20   40  
7   0   5   50   60  
8   18   44   27   45  
9   0   0   0   5  
10   15   52   30   70  
11   50   70   60   70  
12   20   68   22   68  
13   30   40   58   70  
14   0   8   10   35  
15   70   90   70   94  
16   30   30   60   72  
17   0   15   20   40  
18   0   0   0   5  
19
 
0
 
30
 
25
 
60
 


Positive cells, %
CD80
CD86
Patient
-CD40L
+CD40L
-CD40L
+CD40L
1   3   10   7   15  
2   0   0   0   10  
3   5   25   34   55  
4   0   0   80   90  
5   9   20   64   78  
6   0   20   20   40  
7   0   5   50   60  
8   18   44   27   45  
9   0   0   0   5  
10   15   52   30   70  
11   50   70   60   70  
12   20   68   22   68  
13   30   40   58   70  
14   0   8   10   35  
15   70   90   70   94  
16   30   30   60   72  
17   0   15   20   40  
18   0   0   0   5  
19
 
0
 
30
 
25
 
60
 

BCP-ALL cells were cocultured on HBMS in the presence or absence of CD40L transfectants. After 48 hours, the cells were stained with FITC-conjugated mAbs. Phenotypic analysis was performed on the gated cells to exclude cell debris. Results are expressed as percentage of positive cells.

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