Table 2.

FISH and real-time RT-PCR analysis in 48 cases of MM

QRT-PCR
Case
Age
Sex
MG
Histology
Segregation FISH≈
Locus-specific FISH≈
Cyclin D1
MYEOV
IHC Cyclin D1
Group 1          
1   64   M   G,κ   I-LP   NF   ND   666.25   1.99   +++  
2a*  78   F   G,κ   I   Pos   ND   425   9.57   +++  
2b*  78   F   G,κ   I   Pos, ↑ copies   ND   571   ND   +++  
3   58   M   G,κ   I-LP   Pos   ND   408.09   3.3   +++  
4   59   M   NA   I-LP   Pos†, ↑ copies   ND   192.9   3.71   +++  
5   61   F   κ   I   Pos, ↑ copies   ND   175.9   0.55   +++  
6   81   M   λ   I-LP   Pos†, ↑ copies   ND   166.71   0.03   +++  
7   56   F   G,κ   I   Pos†, ↑ copies   ND   163.51   2.71   +++  
8   59   M   κ   I-LP   Pos, ↑ copies   ND   145.22   0.35   +++  
9   76   F   λ   I   Pos   ND   139.24   44.4   +++  
10   40   M   G,κ   I   Pos†, ↑ copies   ND   103.85   1.98   +++  
11*  76   M   κ   I-LP   Pos   ND   96.76   1.79   +++  
Group 2          
12a   67   M   G,κ   I-LP   Neg, ↑ copies   Polysomy   18.72   7.58   ++  
12b   67   M   G,κ   I-LP   Neg, ↑ copies   Polysomy   14.23   ND   ++  
12c   67   M   G,κ   I-LP   Neg, ↑ copies   Polysomy   17.07   ND   +  
13   78   M   NA   I   Neg   Disomy   15.44   ND   ++  
14   69   F   G,κ   I   Neg   Disomy   15.34   24.28   ++  
15‡  32   M   G,κ   I   Neg, ↑ copies   Polysomy   15.36   31.59   +  
16   66   M   G,κ   I-LP   Neg, ↑ copies   Polysomy   12.42   1.76   +  
17a   59   M   G,κ   I   ND   Polysomy   10.49   7.25   +  
17b   59   M   G,κ   I   Neg, ↑ copies   Polysomy   5.27   ND   +  
17c   59   M   G,κ   I   Neg, ↑ copies   Polysomy   6.7   ND   +  
18   74   F   G,κ   I   Neg, ↑ copies   Polysomy   3.78   11.34   ++  
19   74   F   G,κ   I   Neg, ↑ copies   Polysomy   7.79   10.42   Neg∥ 
20   60   F   G,λ   II   Neg§  Polysomy   7.64   1.59   Neg  
21   61   M   NA   I   Neg, ↑ copies   Polysomy   6.6   19.95   Neg∥ 
22*  56   F   NA   I-LP   Neg, ↑ copies   Polysomy   4.14   13.94   Neg  
23   60   F   G,κ   I   Neg, ↑ copies   Polysomy   3.63   0.36   Neg  
24   68   M   NA   I   Neg, ↑ copies   Polysomy   3.28   2.34   Neg∥ 
25*  69   F   G,κ   I   Neg§  Polysomy   2.56   3.57   Neg  
26   73   M   G,κ   I   Neg, ↑ copies   Polysomy   2.5   3.2   Neg  
Group 3          
27   76   M   A,λ   II   Neg   ND   2.03   0.36   Neg  
28   48   F   λ   II   Neg   ND   1.55   0.18   Neg  
29   63   F   G,κ   II   Neg   ND   1.26   NF   Neg  
30   79   F   NA   I   Neg   ND   1.23   2.13   Neg  
31   47   F   κ   I   Neg   ND   1.0   4.23   Neg  
32   71   F   G,κ   I   Neg   ND   0.87   6.6   ND  
33   52   M   G,λ   I   Neg   ND   0.77   Absent   Neg  
34   72   F   NA   II   Neg   ND   0.77   0.44   ND  
35   75   M   NA   II   Neg, ↑ copies   Polysomy   0.67   0.01   Neg  
36   74   F   κ   I   Neg   ND   0.62   0.8   Neg  
37   55   F   λ   I   Neg   ND   0.57   1.14   Neg  
38*  63   F   A,λ   I   Neg   ND   0.46   13.38   Neg  
39a   75   F   κ   I   Neg   ND   0.44   ND   Neg  
39b   75   F   κ   I   Neg   ND   0.14   2.48   Neg  
40   62   F   M,λ   II   Neg   ND   0.7   2.66   Neg  
41   69   M   G,λ   I   Neg   ND   0.38   ND   Neg  
42a   64   M   G,κ   II   Neg   ND   0.37   ND   Neg  
42b   64   M   G,κ   II   Neg   ND   0.82   3.42   Neg  
42c   64   M   G,κ   II   Neg   ND   0.42   ND   Neg  
43   54   M   λ   I-LP   Neg   ND   0.27   4.14   Neg  
44‡  64   F   G,λ   I   Neg   ND   0.26   6.8   Neg  
45   63   M   G,λ   I   Neg   ND   0.26   6.24   Neg  
46   64   F   G,λ   I   Neg   ND   0.2   5.8   Neg  
47   76   M   G,κ   II   Neg   ND   0.13   1.28   Neg  
48
 		 57
 		 M
 		 NA
 		 I
 		 Neg
 		 ND
 		 0.11
 		 0.21
 		 Neg
 
QRT-PCR
Case
Age
Sex
MG
Histology
Segregation FISH≈
Locus-specific FISH≈
Cyclin D1
MYEOV
IHC Cyclin D1
Group 1          
1   64   M   G,κ   I-LP   NF   ND   666.25   1.99   +++  
2a*  78   F   G,κ   I   Pos   ND   425   9.57   +++  
2b*  78   F   G,κ   I   Pos, ↑ copies   ND   571   ND   +++  
3   58   M   G,κ   I-LP   Pos   ND   408.09   3.3   +++  
4   59   M   NA   I-LP   Pos†, ↑ copies   ND   192.9   3.71   +++  
5   61   F   κ   I   Pos, ↑ copies   ND   175.9   0.55   +++  
6   81   M   λ   I-LP   Pos†, ↑ copies   ND   166.71   0.03   +++  
7   56   F   G,κ   I   Pos†, ↑ copies   ND   163.51   2.71   +++  
8   59   M   κ   I-LP   Pos, ↑ copies   ND   145.22   0.35   +++  
9   76   F   λ   I   Pos   ND   139.24   44.4   +++  
10   40   M   G,κ   I   Pos†, ↑ copies   ND   103.85   1.98   +++  
11*  76   M   κ   I-LP   Pos   ND   96.76   1.79   +++  
Group 2          
12a   67   M   G,κ   I-LP   Neg, ↑ copies   Polysomy   18.72   7.58   ++  
12b   67   M   G,κ   I-LP   Neg, ↑ copies   Polysomy   14.23   ND   ++  
12c   67   M   G,κ   I-LP   Neg, ↑ copies   Polysomy   17.07   ND   +  
13   78   M   NA   I   Neg   Disomy   15.44   ND   ++  
14   69   F   G,κ   I   Neg   Disomy   15.34   24.28   ++  
15‡  32   M   G,κ   I   Neg, ↑ copies   Polysomy   15.36   31.59   +  
16   66   M   G,κ   I-LP   Neg, ↑ copies   Polysomy   12.42   1.76   +  
17a   59   M   G,κ   I   ND   Polysomy   10.49   7.25   +  
17b   59   M   G,κ   I   Neg, ↑ copies   Polysomy   5.27   ND   +  
17c   59   M   G,κ   I   Neg, ↑ copies   Polysomy   6.7   ND   +  
18   74   F   G,κ   I   Neg, ↑ copies   Polysomy   3.78   11.34   ++  
19   74   F   G,κ   I   Neg, ↑ copies   Polysomy   7.79   10.42   Neg∥ 
20   60   F   G,λ   II   Neg§  Polysomy   7.64   1.59   Neg  
21   61   M   NA   I   Neg, ↑ copies   Polysomy   6.6   19.95   Neg∥ 
22*  56   F   NA   I-LP   Neg, ↑ copies   Polysomy   4.14   13.94   Neg  
23   60   F   G,κ   I   Neg, ↑ copies   Polysomy   3.63   0.36   Neg  
24   68   M   NA   I   Neg, ↑ copies   Polysomy   3.28   2.34   Neg∥ 
25*  69   F   G,κ   I   Neg§  Polysomy   2.56   3.57   Neg  
26   73   M   G,κ   I   Neg, ↑ copies   Polysomy   2.5   3.2   Neg  
Group 3          
27   76   M   A,λ   II   Neg   ND   2.03   0.36   Neg  
28   48   F   λ   II   Neg   ND   1.55   0.18   Neg  
29   63   F   G,κ   II   Neg   ND   1.26   NF   Neg  
30   79   F   NA   I   Neg   ND   1.23   2.13   Neg  
31   47   F   κ   I   Neg   ND   1.0   4.23   Neg  
32   71   F   G,κ   I   Neg   ND   0.87   6.6   ND  
33   52   M   G,λ   I   Neg   ND   0.77   Absent   Neg  
34   72   F   NA   II   Neg   ND   0.77   0.44   ND  
35   75   M   NA   II   Neg, ↑ copies   Polysomy   0.67   0.01   Neg  
36   74   F   κ   I   Neg   ND   0.62   0.8   Neg  
37   55   F   λ   I   Neg   ND   0.57   1.14   Neg  
38*  63   F   A,λ   I   Neg   ND   0.46   13.38   Neg  
39a   75   F   κ   I   Neg   ND   0.44   ND   Neg  
39b   75   F   κ   I   Neg   ND   0.14   2.48   Neg  
40   62   F   M,λ   II   Neg   ND   0.7   2.66   Neg  
41   69   M   G,λ   I   Neg   ND   0.38   ND   Neg  
42a   64   M   G,κ   II   Neg   ND   0.37   ND   Neg  
42b   64   M   G,κ   II   Neg   ND   0.82   3.42   Neg  
42c   64   M   G,κ   II   Neg   ND   0.42   ND   Neg  
43   54   M   λ   I-LP   Neg   ND   0.27   4.14   Neg  
44‡  64   F   G,λ   I   Neg   ND   0.26   6.8   Neg  
45   63   M   G,λ   I   Neg   ND   0.26   6.24   Neg  
46   64   F   G,λ   I   Neg   ND   0.2   5.8   Neg  
47   76   M   G,κ   II   Neg   ND   0.13   1.28   Neg  
48
 		 57
 		 M
 		 NA
 		 I
 		 Neg
 		 ND
 		 0.11
 		 0.21
 		 Neg
 

Group 1: t(11;14)-positive and high cyclin D1 mRNA and protein. Group 2: t(11;14)-negative and low to intermediate cyclin D1 mRNA and protein. Group 3: t(11;14) and cyclin D1 mRNA and protein negative. MG indicates monoclonal gammopathy; Segregation FISH, breakpoint analysis; Locus-specific FISH, analysis of chromosome copy numbers; IHC, immunohistochemistry; I-LP, Bartl grade I, lymphoplasmacytoid morphology lymphoplasmocytoid morphology; NF, not feasible; ND, not done; +++, ≥ 80% tumor cells strongly positive; I, Bartl grade I; II, Bartl grade II; Pos, positive; ↑ copies, increased copies; NA, data are not available; Neg, negative; ++, 20%-50% tumor cells positive; and +, 10%-20% tumor cells positive.

*

Positive for CD20 in a proportion of tumor cells.

†

Segregation FISH pattern indicative of a translocation with subsequent loss of the derivative chromosome containing MYEOV (see Figure 1D for a representative example).

‡

Multiple biopsies were analyzed.

§

Not informative concerning copy number changes.

∥

Cases were considered negative, however, rare bona fide plasma cells were positive for cyclin D1.

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