Primary antibodies used for immunostaining
Human specificity . | Designation . | Working concentration . | Specification . | Source . |
|---|---|---|---|---|
| Caveolin | C13630 | 2.5 μg/mL | Rabbit | Transduction Laboratories, Lexington, KY |
| Eotaxin-3 | 115002 | 1 μg/mL | Mouse IgG1 | R&D Systems, Abingdon, United Kingdom |
| Eotaxin-3 | BAF346 | 10 μg/mL | Goat IgG | R&D Systems |
| Eotaxin-3 | 500-P156 | 5 μg/mL | Goat | Peprotech, Rocky Hill, NJ |
| Golgi 58K-9 protein | 58-K9 | 1:200 | Mouse IgG1 | Sigma, St Louis, MO |
| GROα | Part 840255 (DuoSet) | 1 μg/mL | Mouse IgG | R&D Systems |
| GROα | BAF275 | 10 μg/mL | Goat IgG | R&D Systems |
| GROα | 500-P92 | 5 μg/mL | Rabbit | Peprotech |
| IL-8 | 4G9 | 1 μg/mL | Mouse IgG1 | Dr. A. Rot, Novartis, Austria |
| IP-10 | 33036.211 | 1 μg/mL | Mouse IgG1 | R&D Systems |
| IP-10 | RDI-IP-10abm | 1 μg/mL | Mouse IgG1 | RDI, Flanders, NJ |
| IP-10 | BAF266 | 10 μg/mL | Goat IgG | R&D Systems |
| EEA1 | 14 | 1.2 μg/mL | Mouse IgG1 | Transduction Laboratories |
| LAMP2 | H4B4 | 1:200 | Mouse IgG1 | Dr J.T. August and Dr J.E.K. Hildreth, John Hopkins, Baltimore, MD |
| LBPA | 6C4 | 1:100 | Mouse IgG | Dr J. Gruenberg, University of Geneva, Switzerland |
| MCP-1 | 23007.111 | 1 μg/mL | Mouse IgG2B | R&D Systems |
| MCP-1 | LS1 D9 | 1 μg/mL | Mouse IgG1 | LeukoSite, Cambridge, MA |
| MCP-1 | BAF279 | 10 μg/mL | Goat IgG | R&D Systems |
| MCP-1 | 500-P34 | 5 μg/mL | Rabbit | Peprotech |
| RANTES | ID2/A12 | 1:200 | Mouse IgG | Dr M. Sticherling, Klinikum der Christian-Albrechts-Universität zu Kiel, Germany |
| RANTES | 21445.1 | 1 μg/mL | Mouse IgG1 | R&D Systems |
| RANTES | 500P-36 | 10 μg/mL | Rabbit | Peprotech |
| RANTES | BAF278 | 5 μg/mL | Goat IgG | R&D Systems |
| VWF | F8/86 | 1:200 | Mouse IgG1 | Dako, Glostrup, Denmark |
| VWF | R0156 | 1:1400 | Rabbit | Dako |
| Irrelevant control | MOPC-21 | — | Mouse IgG1 | Sigma |
Human specificity . | Designation . | Working concentration . | Specification . | Source . |
|---|---|---|---|---|
| Caveolin | C13630 | 2.5 μg/mL | Rabbit | Transduction Laboratories, Lexington, KY |
| Eotaxin-3 | 115002 | 1 μg/mL | Mouse IgG1 | R&D Systems, Abingdon, United Kingdom |
| Eotaxin-3 | BAF346 | 10 μg/mL | Goat IgG | R&D Systems |
| Eotaxin-3 | 500-P156 | 5 μg/mL | Goat | Peprotech, Rocky Hill, NJ |
| Golgi 58K-9 protein | 58-K9 | 1:200 | Mouse IgG1 | Sigma, St Louis, MO |
| GROα | Part 840255 (DuoSet) | 1 μg/mL | Mouse IgG | R&D Systems |
| GROα | BAF275 | 10 μg/mL | Goat IgG | R&D Systems |
| GROα | 500-P92 | 5 μg/mL | Rabbit | Peprotech |
| IL-8 | 4G9 | 1 μg/mL | Mouse IgG1 | Dr. A. Rot, Novartis, Austria |
| IP-10 | 33036.211 | 1 μg/mL | Mouse IgG1 | R&D Systems |
| IP-10 | RDI-IP-10abm | 1 μg/mL | Mouse IgG1 | RDI, Flanders, NJ |
| IP-10 | BAF266 | 10 μg/mL | Goat IgG | R&D Systems |
| EEA1 | 14 | 1.2 μg/mL | Mouse IgG1 | Transduction Laboratories |
| LAMP2 | H4B4 | 1:200 | Mouse IgG1 | Dr J.T. August and Dr J.E.K. Hildreth, John Hopkins, Baltimore, MD |
| LBPA | 6C4 | 1:100 | Mouse IgG | Dr J. Gruenberg, University of Geneva, Switzerland |
| MCP-1 | 23007.111 | 1 μg/mL | Mouse IgG2B | R&D Systems |
| MCP-1 | LS1 D9 | 1 μg/mL | Mouse IgG1 | LeukoSite, Cambridge, MA |
| MCP-1 | BAF279 | 10 μg/mL | Goat IgG | R&D Systems |
| MCP-1 | 500-P34 | 5 μg/mL | Rabbit | Peprotech |
| RANTES | ID2/A12 | 1:200 | Mouse IgG | Dr M. Sticherling, Klinikum der Christian-Albrechts-Universität zu Kiel, Germany |
| RANTES | 21445.1 | 1 μg/mL | Mouse IgG1 | R&D Systems |
| RANTES | 500P-36 | 10 μg/mL | Rabbit | Peprotech |
| RANTES | BAF278 | 5 μg/mL | Goat IgG | R&D Systems |
| VWF | F8/86 | 1:200 | Mouse IgG1 | Dako, Glostrup, Denmark |
| VWF | R0156 | 1:1400 | Rabbit | Dako |
| Irrelevant control | MOPC-21 | — | Mouse IgG1 | Sigma |
—indicates variable.