Table 1.

Release of T-cell cytokines into supernatants of memory cell pool cultures during 6 days of culture with 10 ng/mL FVIII in the presence or absence of blocking antibodies or appropriate isotype-specific control antibodies (control)

Concentrations of cytokines in supernatants, pg/mL
Culture conditions
IL-2
IL-4
IL-5
IL-6
IL-10
IFN-γ
FVIII only   12.1   3.7   10.2   13.4   636.1   358.2  
FVIII + α-CD80   26.5   1.0   ND   ND   65.8   91.5  
FVIII + control   11.7   0.7   9.5   16.5   506.9   242.6  
FVIII + α-CD86   9.5   ND   ND   ND   73.9   39.8  
FVIII + control   9.3   ND   7.8   12.8   498.7   386.5  
FVIII + α-CD80/86   ND   ND   ND   ND   11.5   ND  
FVIII + control   7.7   ND   10.6   13.5   566.5   344.6  
FVIII + α-CD40L   13.2   ND   10.4   12.5   261.8   125.2  
FVIII + control   11.7   0.7   9.5   16.5   506.9   242.6  
FVIII + α-ICOSL   14.7   10.2   12.4   14.5   634.1   293.7  
FVIII + control
 		 10.8
 		 11.9
 		 11.6
 		 17.8
 		 707.5
 		 326.5
 
Concentrations of cytokines in supernatants, pg/mL
Culture conditions
IL-2
IL-4
IL-5
IL-6
IL-10
IFN-γ
FVIII only   12.1   3.7   10.2   13.4   636.1   358.2  
FVIII + α-CD80   26.5   1.0   ND   ND   65.8   91.5  
FVIII + control   11.7   0.7   9.5   16.5   506.9   242.6  
FVIII + α-CD86   9.5   ND   ND   ND   73.9   39.8  
FVIII + control   9.3   ND   7.8   12.8   498.7   386.5  
FVIII + α-CD80/86   ND   ND   ND   ND   11.5   ND  
FVIII + control   7.7   ND   10.6   13.5   566.5   344.6  
FVIII + α-CD40L   13.2   ND   10.4   12.5   261.8   125.2  
FVIII + control   11.7   0.7   9.5   16.5   506.9   242.6  
FVIII + α-ICOSL   14.7   10.2   12.4   14.5   634.1   293.7  
FVIII + control
 		 10.8
 		 11.9
 		 11.6
 		 17.8
 		 707.5
 		 326.5
 

Results were obtained in a representative experiment. ND indicates not detectable (below detection limit).

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