Reactivity of HLA-A-specific humAbs with a panel of HLA-typed peripheral blood lymphocytes and cell lines
mAb . | Isotype . | Specificity . | PBLs . | Cell lines . | Flow cytometry . |
|---|---|---|---|---|---|
| VDK1D12 | IgM, κ | A1 | 10* | 2* | +++ |
| SN66E3 | IgM, κ | A2 | 22 | 3 | ++/+++ |
| SN66E3 | IgM, κ | A28 | 3 | 0 | +++ |
| OK2F3 | IgM, κ | A3 | 11 | 2 | +++ |
| GV5D1 | IgG1, λ | A1 | 10 | 2 | +++ |
| GV5D1 | IgG1, λ | A9 | 8 | 0 | +++ |
| NIE44B8 | IgM, κ | A10 | 4 | 1 | +/++ |
| BRO11F6 | IgG1, λ | A11 | 4δ | 3 | -/+++ |
| HDG6B6 | IgM, λ | A29, 31, 32, 33 (19) | 12 | 2 | + |
| SN230G6 | IgG1, λ | A2 | 22 | 3 | +++ |
| OK5A3 | IgM, λ | A3 | 8 | 2 | +++ |
| OK5A3 | IgM, λ | A11 | 3 | 3 | + |
| OK5A3 | IgM, λ | A1 | 10 | 2 | + |
| OK4F9 | IgM, κ | A1 | 8 | 2 | +++ |
| OK4F9 | IgM, κ | A3 | 7 | 2 | +++ |
| OK4F9 | IgM, κ | A11 | 3 | 3 | +++ |
| HDG2G7 | IgG1, κ | A29, 31, 32, 33 (19) | 16 | 2 | +++ |
| OK1C9 | IgM, λ | A3 | 11 | 2 | +++ |
| OK1C9 | IgM, λ | A11 | 3 | 2 | + |
| OK1C9 | IgM, λ | A33 (19) | 2 | 0 | + |
| OK1C9 | IgM, λ | A31 (19) | 2 | 1 | + |
mAb . | Isotype . | Specificity . | PBLs . | Cell lines . | Flow cytometry . |
|---|---|---|---|---|---|
| VDK1D12 | IgM, κ | A1 | 10* | 2* | +++ |
| SN66E3 | IgM, κ | A2 | 22 | 3 | ++/+++ |
| SN66E3 | IgM, κ | A28 | 3 | 0 | +++ |
| OK2F3 | IgM, κ | A3 | 11 | 2 | +++ |
| GV5D1 | IgG1, λ | A1 | 10 | 2 | +++ |
| GV5D1 | IgG1, λ | A9 | 8 | 0 | +++ |
| NIE44B8 | IgM, κ | A10 | 4 | 1 | +/++ |
| BRO11F6 | IgG1, λ | A11 | 4δ | 3 | -/+++ |
| HDG6B6 | IgM, λ | A29, 31, 32, 33 (19) | 12 | 2 | + |
| SN230G6 | IgG1, λ | A2 | 22 | 3 | +++ |
| OK5A3 | IgM, λ | A3 | 8 | 2 | +++ |
| OK5A3 | IgM, λ | A11 | 3 | 3 | + |
| OK5A3 | IgM, λ | A1 | 10 | 2 | + |
| OK4F9 | IgM, κ | A1 | 8 | 2 | +++ |
| OK4F9 | IgM, κ | A3 | 7 | 2 | +++ |
| OK4F9 | IgM, κ | A11 | 3 | 3 | +++ |
| HDG2G7 | IgG1, κ | A29, 31, 32, 33 (19) | 16 | 2 | +++ |
| OK1C9 | IgM, λ | A3 | 11 | 2 | +++ |
| OK1C9 | IgM, λ | A11 | 3 | 2 | + |
| OK1C9 | IgM, λ | A33 (19) | 2 | 0 | + |
| OK1C9 | IgM, λ | A31 (19) | 2 | 1 | + |
HLA-A allele–specific mAbs were tested with HLA class I–typed peripheral blood lymphocytes (PBLs) and cell lines in indirect immunofluorescence (IIF). Two isotype-matched controls were included in every experiment. With the exception of mAb BRO11F6, all mAbs stained all cell samples expressing the corresponding HLA class I allospecificity. Reactivity is expressed as 1 log (+), 2 logs (++), or 3 logs (+++) stronger than the negative controls used. - indicates negative reaction. Numbers in parentheses indicate broad specificity.
Number of samples tested. - indicates negative reaction. Numbers in parentheses indicate broad specificity.
mAb BRO11F6 stained only 2 of the 4 HLA-A 1104–positive PBL samples.