Table 1.

Induction of MDR-1, CD25, and CD122 mRNA by depsipeptide

Depsipeptide, ng/mL
MDR-1
IL-2Rα
IL-2Rβ
24 h
48 h
24 h
48 h
24 h
48 h
0   1   1   1   1   1   1  
1   4.62   7.66   19.33   1.45   1.8   2.4  
2
 		 18.47
 		 31.03
 		 10.19
 		 0.65
 		 2
 		 2.81
 
Depsipeptide, ng/mL
MDR-1
IL-2Rα
IL-2Rβ
24 h
48 h
24 h
48 h
24 h
48 h
0   1   1   1   1   1   1  
1   4.62   7.66   19.33   1.45   1.8   2.4  
2
 		 18.47
 		 31.03
 		 10.19
 		 0.65
 		 2
 		 2.81
 

HUT78 cells were treated for 24 to 48 hours with 0, 1, 2, or 4 ng/mL of depsipeptide, as indicated, and RNA was isolated using RNeasy. RT-PCR was performed as described in “Materials and methods.” Values represent the fold induction of mRNA detected by RT-PCR after normalization by the RT-PCR product of rRNA.

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