Sequences of the primers used for screening of HJV mutations
Primer . | Sequence . | PCR product length, bp . |
|---|---|---|
| Exon 1 F | 5′ CCGTCAACTCAGTAGCCA 3′ | 336 |
| Exon 1 R | 5′ CATTTGGACGAGAGACATC 3′ | |
| Exon 2 F | 5′ CCCCAAATTCCAGTCTGTT 3′ | 380 |
| Exon 2 R | 5′ CTCATTCAGGCTCACATGC 3′ | |
| Exon 3 F | 5′ CTCCGATAGAGCAGAGGTC 3′ | 709 |
| Exon 3 R | 5′ GGAGCATTGCTGTTGAATAG 3′ | |
| Exon 4a F | 5′ ACTATGTATGAGGTCTGATTG 3′ | 840 |
| Exon 4a R | 5′ CTGCAGCCTCATCTGACTC 3′ | |
| Exon 4b F | 5′ CAGCTGAACAGGACCTGC3′ | 1043 |
| Exon 4b R | 5′ CACATTGCATTTACTCCATTTC 3′ |
Primer . | Sequence . | PCR product length, bp . |
|---|---|---|
| Exon 1 F | 5′ CCGTCAACTCAGTAGCCA 3′ | 336 |
| Exon 1 R | 5′ CATTTGGACGAGAGACATC 3′ | |
| Exon 2 F | 5′ CCCCAAATTCCAGTCTGTT 3′ | 380 |
| Exon 2 R | 5′ CTCATTCAGGCTCACATGC 3′ | |
| Exon 3 F | 5′ CTCCGATAGAGCAGAGGTC 3′ | 709 |
| Exon 3 R | 5′ GGAGCATTGCTGTTGAATAG 3′ | |
| Exon 4a F | 5′ ACTATGTATGAGGTCTGATTG 3′ | 840 |
| Exon 4a R | 5′ CTGCAGCCTCATCTGACTC 3′ | |
| Exon 4b F | 5′ CAGCTGAACAGGACCTGC3′ | 1043 |
| Exon 4b R | 5′ CACATTGCATTTACTCCATTTC 3′ |