Table 1. Changes in size and cellularity... | American Society of Hematology

Table 1.

Changes in size and cellularity of spleens and numbers of splenic erythroblasts of naive or P chabaudi AS-infected mice after EPO treatment

	N*	N + EPO†	I*	I + EPO†
Spleen index, %	0.29 ± 0.01^a	0.87 ± 0.01^b	1.34 ± 0.08^c	1.43 ± 0.07^c
Cellularity, × 10⁶ cells	25.00 ± 2.52^a	160.50 ± 5.74^b	180.40 ± 27.26^b	178.00 ± 19.34^b
TER119⁺, × 10⁶ cells	0.51 ± 0.08^a	36.33 ± 2.05^b	14.53 ± 2.62^c	10.87 ± 0.93^c
Hb-positive, × 10⁶ cells	0.17 ± 0.01^a	27.24 ± 0.62^b	4.17 ± 1.02^c	3.97 ± 0.15^c
Hb-positive/TER119⁺‡	34.67 ± 6.41^a	74.99 ± 4.81^b	29.21 ± 3.84^a	30.18 ± 2.91^a

	N*	N + EPO†	I*	I + EPO†
Spleen index, %	0.29 ± 0.01^a	0.87 ± 0.01^b	1.34 ± 0.08^c	1.43 ± 0.07^c
Cellularity, × 10⁶ cells	25.00 ± 2.52^a	160.50 ± 5.74^b	180.40 ± 27.26^b	178.00 ± 19.34^b
TER119⁺, × 10⁶ cells	0.51 ± 0.08^a	36.33 ± 2.05^b	14.53 ± 2.62^c	10.87 ± 0.93^c
Hb-positive, × 10⁶ cells	0.17 ± 0.01^a	27.24 ± 0.62^b	4.17 ± 1.02^c	3.97 ± 0.15^c
Hb-positive/TER119⁺‡	34.67 ± 6.41^a	74.99 ± 4.81^b	29.21 ± 3.84^a	30.18 ± 2.91^a

Data are means ± SEM for 4 to 5 mice per group. ANOVA in conjunction with Tukey post-hoc analysis was employed to analyze statistical differences among data from N, N+EPO, I, and I+EPO. Results of statistical comparison are indicated by lowercase letter superscripts assigned to each set of data. When 2 sets of data are labeled with superscripts of different lowercase letters, it indicates that the 2 sets of data are statistically different (P < .05). Similar results were obtained in 3 independent experiments.

EPO indicates erythropoietin; N, naive mice; N + EPO, naive mice treated with EPO; I, infected mice; I + EPO, infected mice treated with EPO; Hb, hemoglobin.

Spleens from naive and P chabaudi AS-infected A/J mice without treatment or treated with 0.1% BSA solution were used as controls. No significant differences between spleens from untreated mice or 0.1% BSA-treated mice were observed. Therefore, only data from untreated mice are reported here.

†

Naive or infected A/J mice were treated with 10 units of rmEPO per day for 3 consecutive days. Mice were humanely killed 1 day following the completion of the treatment, and spleen parameters were assessed.

‡

Data were calculated by dividing the number of Hb-positive cells by the number of TER119⁺ cells and multiplying by 100%.

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