Increased megakaryocyte numbers in Aurora-B/AIM-1 transgenic mice were not accompanied by augmented platelet levels
. | Acetylcholinesterase-positive cells . | CD41+ cells . | . | |
|---|---|---|---|---|
| Line . | Per 106 cytospun bone marrow cells . | . | Platelet number × 103/mL . | |
| Wild-type | 677 ± 132 (n = 6) | 657 ± 122 (n = 6) | 12 539 ± 3387 (n = 48) | |
| TG58 | 850 ± 151 (n = 6) | 877 ± 156 (n = 6) | 13 065 ± 2896 (n = 19) | |
| TG74 | 921 ± 136 (n = 6) | 832 ± 152 (n = 6) | 14 325 ± 2703 (n = 15) | |
. | Acetylcholinesterase-positive cells . | CD41+ cells . | . | |
|---|---|---|---|---|
| Line . | Per 106 cytospun bone marrow cells . | . | Platelet number × 103/mL . | |
| Wild-type | 677 ± 132 (n = 6) | 657 ± 122 (n = 6) | 12 539 ± 3387 (n = 48) | |
| TG58 | 850 ± 151 (n = 6) | 877 ± 156 (n = 6) | 13 065 ± 2896 (n = 19) | |
| TG74 | 921 ± 136 (n = 6) | 832 ± 152 (n = 6) | 14 325 ± 2703 (n = 15) | |
All values are mean ± SD for the number of mice indicated in parentheses.
Bone marrow cells (4 × 105) isolated from wild-type or transgenic (TG58, TG74) mice were cytospun onto microscope slides and fixed. Cells were stained with fluorescence-labeled anti-CD41 antibody or were assayed for acetylcholinesterase activity (both are markers for murine megakaryocytes). Positively stained cells were scored under the microscope. An increase of approximately 25% to 30% occurred in megakaryocyte numbers in transgenic lines compared with wild-type mice (P < .01 in each pair; Student t test). Platelet number was determined in the blood of wild-type or transgenic mice, as described in “Materials and methods.”