Oligonucleotide sequences for PCR
Gene (GenBank accession no.) . | Position, nt . | Primer sequence . |
|---|---|---|
| AGF (AB054065) | 918 to 1089 | 5′-TCGTGTAGTAGCCGTGTGGTGT-3′ |
| 5′-CACCTGATGCACAGGTTCCA-3′ | ||
| bFGF (NM_008006) | 163 to 213 | 5′-AGCGACCCACACGTCAAACT-3′ |
| 5′-CACAACTCCTCTCTCTTCTGCTTG-3′ | ||
| VEGF (NM_009505) | 180 to 230 | 5′-GGAGAGCAGAAGTCCCATGAAGT-3′ |
| 5′-TCGCTGGTAGACGTCCATGA-3′ | ||
| VEGF12021 | 5′-CATAGAGAGAATGAGCTTCCTACAGC-3′ | |
| 5′-CCTTGGCTTGTCACATTTTTC-3′ | ||
| VEGF164 (NM_009505) | 464 to 529 | 5′-ATGTGAATGCAGACCAAAGAAAGA-3′ |
| 5′-CGCTCTGAACAAGGCTCACAG-3′ | ||
| VEGF18821 | 5′-GGTTTAAATCCTGGAGCGTTCA-3′ | |
| 5′-CAGGAACATTTACACGTCTGCG-3′ | ||
| PIGF (NM_008827) | 719 to 809 | 5′-ACGACAAAGGCAGAAAGGAGG-3′ |
| 5′-GGAAATGACATCACGGGTGG-3′ | ||
| Ang-1 (NM_009640) | 587 to 677 | 5′-CTCAGTGGCTGCAAAAACTTGA-3′ |
| 5′-GTGTGGTTTTGAACAGCATTCTG-3′ | ||
| GAPDH (NM_008084) | 119 to 222 | 5′-AAAGTGGAGATTGTTGCCAT-3′ |
| 5′-TTGACTGTGCCGTTGAATT-3′ |
Gene (GenBank accession no.) . | Position, nt . | Primer sequence . |
|---|---|---|
| AGF (AB054065) | 918 to 1089 | 5′-TCGTGTAGTAGCCGTGTGGTGT-3′ |
| 5′-CACCTGATGCACAGGTTCCA-3′ | ||
| bFGF (NM_008006) | 163 to 213 | 5′-AGCGACCCACACGTCAAACT-3′ |
| 5′-CACAACTCCTCTCTCTTCTGCTTG-3′ | ||
| VEGF (NM_009505) | 180 to 230 | 5′-GGAGAGCAGAAGTCCCATGAAGT-3′ |
| 5′-TCGCTGGTAGACGTCCATGA-3′ | ||
| VEGF12021 | 5′-CATAGAGAGAATGAGCTTCCTACAGC-3′ | |
| 5′-CCTTGGCTTGTCACATTTTTC-3′ | ||
| VEGF164 (NM_009505) | 464 to 529 | 5′-ATGTGAATGCAGACCAAAGAAAGA-3′ |
| 5′-CGCTCTGAACAAGGCTCACAG-3′ | ||
| VEGF18821 | 5′-GGTTTAAATCCTGGAGCGTTCA-3′ | |
| 5′-CAGGAACATTTACACGTCTGCG-3′ | ||
| PIGF (NM_008827) | 719 to 809 | 5′-ACGACAAAGGCAGAAAGGAGG-3′ |
| 5′-GGAAATGACATCACGGGTGG-3′ | ||
| Ang-1 (NM_009640) | 587 to 677 | 5′-CTCAGTGGCTGCAAAAACTTGA-3′ |
| 5′-GTGTGGTTTTGAACAGCATTCTG-3′ | ||
| GAPDH (NM_008084) | 119 to 222 | 5′-AAAGTGGAGATTGTTGCCAT-3′ |
| 5′-TTGACTGTGCCGTTGAATT-3′ |
nt indicates nucleotide.