Characterization of Ba/F3 cells expressing FLT3-ITD/TKD dual mutants
Ba/F3 cell line . | IC50 of SU5614, μM . | IL-3–independent growth . |
|---|---|---|
| ITD (W51) | 0.2 | + |
| D835N | 0.1 | + |
| ITD-D835N | 1.0 | + |
| Y842H | 0.2 | + |
| ITD-Y842H | 1.1 | + |
Ba/F3 cell line . | IC50 of SU5614, μM . | IL-3–independent growth . |
|---|---|---|
| ITD (W51) | 0.2 | + |
| D835N | 0.1 | + |
| ITD-D835N | 1.0 | + |
| Y842H | 0.2 | + |
| ITD-Y842H | 1.1 | + |
The IC50 of SU5614 in Ba/F3 cell lines expressing either the D835N or Y842H or the FLT3-ITD mutant alone and the FLT3-ITD/D835N, FLT3-ITD/Y842H dual mutants were determined as described in Figure 7. + indicates IL-3–independent growth.