Interference of HIV-1 Tat peptides on chemokine-driven Vδ2 and Vδ1 T-cell migration: titration of the inhibitory activity of the peptides Tat24-51 and Tat20-39
Tat peptide (ng/mL) . | Vδ2 vs IP-10 . | Vδ1 vs SDF-1 . |
|---|---|---|
| Nil | 48 ± 3 | 38 ± 3 |
| 24-51 (500) | 10 ± 2 | 12 ± 2 |
| 24-51 (50) | 29 ± 3 | 10 ± 1 |
| 24-51 (5) | 43 ± 1 | 39 ± 2 |
| 20-39 (500) | 14 ± 3 | 11 ± 1 |
| 20-39 (50) | 36 ± 2 | 31 ± 3 |
| 20-39 (5) | 43 ± 2 | 41 ± 4 |
| 46-60 (500) | 42 ± 1 | 32 ± 2 |
| 56-70 (500) | 39 ± 2 | 40 ± 3 |
| 65-80RGD (500) | 19 ± 3 | 18 ± 2 |
| 65-80RGE (500) | 41 ± 4 | 36 ± 3 |
Tat peptide (ng/mL) . | Vδ2 vs IP-10 . | Vδ1 vs SDF-1 . |
|---|---|---|
| Nil | 48 ± 3 | 38 ± 3 |
| 24-51 (500) | 10 ± 2 | 12 ± 2 |
| 24-51 (50) | 29 ± 3 | 10 ± 1 |
| 24-51 (5) | 43 ± 1 | 39 ± 2 |
| 20-39 (500) | 14 ± 3 | 11 ± 1 |
| 20-39 (50) | 36 ± 2 | 31 ± 3 |
| 20-39 (5) | 43 ± 2 | 41 ± 4 |
| 46-60 (500) | 42 ± 1 | 32 ± 2 |
| 56-70 (500) | 39 ± 2 | 40 ± 3 |
| 65-80RGD (500) | 19 ± 3 | 18 ± 2 |
| 65-80RGE (500) | 41 ± 4 | 36 ± 3 |
γδ T-cell lines, cultured in IL-2-containing medium (25 U/mL) for 3 weeks, were radiolabeled with 51Cr and assayed for migration, in response to IP-10 (50 ng/mL) for Vδ2 or SDF-1 (50 ng/mL) for Vδ1, across HUVEC monolayers using a transwell double-chamber system, in the absence (Nil) or presence of the indicated Tat synthetic peptides (500-5 ng/mL, as indicated). After 60 minutes of transmigration, cells were recovered from the lower chamber, lysed, and counted in a γ-counter. Results are expressed as percentage of migrated cells, mean ± SD from 6 independent experiments for each cell population from 6 donors, calculated as described in “Patients, materials, and methods.”