Expression of CXCR3 or CXCR4 on Vδ1 or Vδ2 T cells in peripheral blood of HIV-1—infected patients and healthy donors
. | Vδ2+ . | Vδ2+ CXCR3+ . | Vδ2+ CXCR4+ . | Vδ1+ . | Vδ1+ CXCR3+ . | Vδ1+ CXCR4+ . |
---|---|---|---|---|---|---|
Patients | ||||||
1 | 1.0 | 0.9 | 0.9 | 1.0 | 0.8 | 1.0 |
2 | 1.6 | 1.5 | 1.6 | 1.2 | 1.0 | 1.2 |
3 | 0.7 | 0.6 | 0.7 | 1.4 | 1.3 | 1.2 |
4 | 1.9 | 1.8 | 1.7 | 2.2 | 1.8 | 2.0 |
5 | 0.7 | 0.7 | 0.7 | 4.2 | 3.2 | 3.6 |
6 | 2.7 | 2.8 | 2.3 | 4.1 | 2.0 | 3.9 |
7 | 0.3 | 0.2 | 0.3 | 1.0 | 0.9 | 1.1 |
8 | 3.8 | 2.8 | 3.1 | 2.2 | 1.6 | 1.8 |
9 | 0.4 | 0.3 | 0.4 | 2.3 | 2.0 | 1.9 |
10 | 2.4 | 2.3 | 2.6 | 2.9 | 1.9 | 2.9 |
11 | 0.3 | 0.2 | 0.3 | 8.8 | 4.3 | 8.6 |
12 | 1.3 | 1.3 | 1.2 | 2.7 | 1.7 | 1.4 |
13 | 1.1 | 1.0 | 1.3 | 2.3 | 1.6 | 2.0 |
14 | 3.0 | 1.9 | 1.6 | 1.2 | 1.0 | 1.2 |
15 | 1.0 | 0.9 | 0.7 | 2.2 | 1.3 | 2.2 |
16 | 3.2 | 2.4 | 2.0 | 4.1 | 2.3 | 3.9 |
17 | 3.5 | 2.2 | 1.0 | 4.2 | 2.0 | 1.7 |
18 | 4.0 | 3.3 | 1.1 | 9.2 | 2.9 | 4.8 |
19 | 2.0 | 1.9 | 1.0 | 0.9 | 0.6 | 0.4 |
20 | 1.8 | 1.4 | 1.3 | 4.4 | 3.3 | 3.3 |
21 | 3.4 | 2.9 | 2.6 | 9.3 | 7.3 | 6.4 |
22 | 1.4 | 1.4 | 1.6 | 2.7 | 1.7 | 2.7 |
23 | 0.4 | 0.2 | 0.2 | 2.0 | 0.8 | 0.6 |
24 | 4.8 | 3.8 | 4.8 | 3.0 | 2.9 | 2.0 |
25 | 3.3 | 2.5 | 3.2 | 1.8 | 1.5 | 1.7 |
26 | 2.2 | 0.8 | 0.4 | 3.6 | 1.2 | 0.7 |
27 | 2.0 | 2.0 | 2.2 | 2.7 | 1.7 | 2.2 |
28 | 1.4 | 0.7 | 1.4 | 4.0 | 2.8 | 3.9 |
Healthy donors | ||||||
1 | 0.9 | 0.8 | 0.2 | 1.9 | 0.8 | 1.6 |
2 | 1.3 | 1.2 | 0.3 | 1.2 | 0.6 | 1.2 |
3 | 3.1 | 2.8 | 0.7 | 0.9 | 0.6 | 0.8 |
4 | 2.9 | 2.2 | 0.6 | 0.6 | 0.2 | 0.5 |
5 | 0.8 | 0.6 | 0.1 | 0.5 | 0.2 | 0.5 |
6 | 1.4 | 1.0 | 0.3 | 2.3 | 0.9 | 2.1 |
7 | 2.6 | 2.2 | 0.6 | 0.8 | 0.2 | 0.6 |
8 | 2.0 | 1.9 | 0.2 | 0.2 | 0.1 | 0.1 |
9 | 2.3 | 2.0 | 0.3 | 2.2 | 1.0 | 2.1 |
10 | 1.6 | 1.2 | 0.9 | 2.0 | 0.9 | 1.9 |
11 | 2.8 | 2.0 | 0.3 | 0.8 | 0.4 | 0.7 |
12 | 1.7 | 1.3 | 0.2 | 1.8 | 1.0 | 1.7 |
13 | 1.8 | 1.0 | 1.1 | 1.7 | 0.9 | 0.6 |
14 | 1.9 | 1.8 | 0.5 | 1.1 | 0.5 | 0.9 |
15 | 2.4 | 1.6 | 0.2 | 0.4 | 0.2 | 0.4 |
. | Vδ2+ . | Vδ2+ CXCR3+ . | Vδ2+ CXCR4+ . | Vδ1+ . | Vδ1+ CXCR3+ . | Vδ1+ CXCR4+ . |
---|---|---|---|---|---|---|
Patients | ||||||
1 | 1.0 | 0.9 | 0.9 | 1.0 | 0.8 | 1.0 |
2 | 1.6 | 1.5 | 1.6 | 1.2 | 1.0 | 1.2 |
3 | 0.7 | 0.6 | 0.7 | 1.4 | 1.3 | 1.2 |
4 | 1.9 | 1.8 | 1.7 | 2.2 | 1.8 | 2.0 |
5 | 0.7 | 0.7 | 0.7 | 4.2 | 3.2 | 3.6 |
6 | 2.7 | 2.8 | 2.3 | 4.1 | 2.0 | 3.9 |
7 | 0.3 | 0.2 | 0.3 | 1.0 | 0.9 | 1.1 |
8 | 3.8 | 2.8 | 3.1 | 2.2 | 1.6 | 1.8 |
9 | 0.4 | 0.3 | 0.4 | 2.3 | 2.0 | 1.9 |
10 | 2.4 | 2.3 | 2.6 | 2.9 | 1.9 | 2.9 |
11 | 0.3 | 0.2 | 0.3 | 8.8 | 4.3 | 8.6 |
12 | 1.3 | 1.3 | 1.2 | 2.7 | 1.7 | 1.4 |
13 | 1.1 | 1.0 | 1.3 | 2.3 | 1.6 | 2.0 |
14 | 3.0 | 1.9 | 1.6 | 1.2 | 1.0 | 1.2 |
15 | 1.0 | 0.9 | 0.7 | 2.2 | 1.3 | 2.2 |
16 | 3.2 | 2.4 | 2.0 | 4.1 | 2.3 | 3.9 |
17 | 3.5 | 2.2 | 1.0 | 4.2 | 2.0 | 1.7 |
18 | 4.0 | 3.3 | 1.1 | 9.2 | 2.9 | 4.8 |
19 | 2.0 | 1.9 | 1.0 | 0.9 | 0.6 | 0.4 |
20 | 1.8 | 1.4 | 1.3 | 4.4 | 3.3 | 3.3 |
21 | 3.4 | 2.9 | 2.6 | 9.3 | 7.3 | 6.4 |
22 | 1.4 | 1.4 | 1.6 | 2.7 | 1.7 | 2.7 |
23 | 0.4 | 0.2 | 0.2 | 2.0 | 0.8 | 0.6 |
24 | 4.8 | 3.8 | 4.8 | 3.0 | 2.9 | 2.0 |
25 | 3.3 | 2.5 | 3.2 | 1.8 | 1.5 | 1.7 |
26 | 2.2 | 0.8 | 0.4 | 3.6 | 1.2 | 0.7 |
27 | 2.0 | 2.0 | 2.2 | 2.7 | 1.7 | 2.2 |
28 | 1.4 | 0.7 | 1.4 | 4.0 | 2.8 | 3.9 |
Healthy donors | ||||||
1 | 0.9 | 0.8 | 0.2 | 1.9 | 0.8 | 1.6 |
2 | 1.3 | 1.2 | 0.3 | 1.2 | 0.6 | 1.2 |
3 | 3.1 | 2.8 | 0.7 | 0.9 | 0.6 | 0.8 |
4 | 2.9 | 2.2 | 0.6 | 0.6 | 0.2 | 0.5 |
5 | 0.8 | 0.6 | 0.1 | 0.5 | 0.2 | 0.5 |
6 | 1.4 | 1.0 | 0.3 | 2.3 | 0.9 | 2.1 |
7 | 2.6 | 2.2 | 0.6 | 0.8 | 0.2 | 0.6 |
8 | 2.0 | 1.9 | 0.2 | 0.2 | 0.1 | 0.1 |
9 | 2.3 | 2.0 | 0.3 | 2.2 | 1.0 | 2.1 |
10 | 1.6 | 1.2 | 0.9 | 2.0 | 0.9 | 1.9 |
11 | 2.8 | 2.0 | 0.3 | 0.8 | 0.4 | 0.7 |
12 | 1.7 | 1.3 | 0.2 | 1.8 | 1.0 | 1.7 |
13 | 1.8 | 1.0 | 1.1 | 1.7 | 0.9 | 0.6 |
14 | 1.9 | 1.8 | 0.5 | 1.1 | 0.5 | 0.9 |
15 | 2.4 | 1.6 | 0.2 | 0.4 | 0.2 | 0.4 |
Direct immunofluorescence on whole blood of HIV-1-infected patients was performed with PE-anti-CXCR3 or -CXCR4 mAbs, and the anti-Vδ1 A13 or the anti-Vδ2 BB3 mAbs were labeled using the Zenon Alexafluor 488 mouse IgG1 Labeling Kit. Samples were analyzed on a flow cytometer equipped with an argon ion laser exciting PE and Alexafluor at 488 nm and gated on lymphocytes. Data are expressed as percentage of positive or double-positive cells.