Sequences of the HEPC primers used in the mutation screening
Primer . | Sequence . | PCR product length, bp . |
|---|---|---|
| Exon 1 | 206 | |
| F | 5′CAAGCTCAAGACCCAGCAGT3′ | |
| R | 5′CAGTGCCCTAGGCTGA3′ | |
| Exon 2 | 192 | |
| F | 5′CAGTCTCAGAGGTCCACT3′ | |
| R | 5′AATGTGAGCAGGGAACC3′ | |
| Exon 3 | 352 | |
| F | 5′CAGTGATGCCTTTCCTAGC3′ | |
| R | 5′AAGGCAGGGTCAGGACAA3′ |
Primer . | Sequence . | PCR product length, bp . |
|---|---|---|
| Exon 1 | 206 | |
| F | 5′CAAGCTCAAGACCCAGCAGT3′ | |
| R | 5′CAGTGCCCTAGGCTGA3′ | |
| Exon 2 | 192 | |
| F | 5′CAGTCTCAGAGGTCCACT3′ | |
| R | 5′AATGTGAGCAGGGAACC3′ | |
| Exon 3 | 352 | |
| F | 5′CAGTGATGCCTTTCCTAGC3′ | |
| R | 5′AAGGCAGGGTCAGGACAA3′ |