Primers used to amplify CD18 cDNA as 4 overlapping fragments
Fragment . | Primer sequence . |
|---|---|
| 1 | |
| CD 18/178F | 5′ - ACT GGC GGC CGC ATG CTG GGC CTG CGC CCC CCA - 3′ |
| CD 18/784R | 5′ - CAA ACG GGG GCT GGC ACT CT - 3′ |
| 2 | |
| CD 18/483F | 5′ - AGT GAC GCT TTA CCT GCG ACC A - 3′ |
| CD 18/1188R | 5′ - GAT GAT CTC GGT GAG TTT CTC GTA G - 3′ |
| 3 | |
| CD 18/1032F | 5′ - CTG TCA CCT GGA GGA CAA CTT GTA C - 3′ |
| CD 18/1916R | 5′ - TCA GTG GTC CTC TCG CAC TG - 3′ |
| 4 | |
| CD 18/1738F | 5′ - ACC AGC GAC GTC CCC GGC AA - 3′ |
| CD 18/2487R | 5′ - CAT GCT CGA GCT AAC TCT CAG CAA ACT TGG G - 3′ |
Fragment . | Primer sequence . |
|---|---|
| 1 | |
| CD 18/178F | 5′ - ACT GGC GGC CGC ATG CTG GGC CTG CGC CCC CCA - 3′ |
| CD 18/784R | 5′ - CAA ACG GGG GCT GGC ACT CT - 3′ |
| 2 | |
| CD 18/483F | 5′ - AGT GAC GCT TTA CCT GCG ACC A - 3′ |
| CD 18/1188R | 5′ - GAT GAT CTC GGT GAG TTT CTC GTA G - 3′ |
| 3 | |
| CD 18/1032F | 5′ - CTG TCA CCT GGA GGA CAA CTT GTA C - 3′ |
| CD 18/1916R | 5′ - TCA GTG GTC CTC TCG CAC TG - 3′ |
| 4 | |
| CD 18/1738F | 5′ - ACC AGC GAC GTC CCC GGC AA - 3′ |
| CD 18/2487R | 5′ - CAT GCT CGA GCT AAC TCT CAG CAA ACT TGG G - 3′ |
The numbering of nucleotides is based on sequence data provided by Weitzman et al,12 with the initiating ATG beginning with A178. Underlined nucleotides in the primers for fragments 1 and 4 are Not I and Xhol linkers, respectively (boldface), plus 4 additional random nucleotides added to enhance product stability (plain type).