Summary of rhesus monkey transplants
. | . | Drug treatment . | . | . | . | . | . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Monkey . | Vector . | Day after transplantation . | Drug doses, mg/kg × d . | SCF + G-CSF before treatment . | Days ANC was below 500 . | Pre-Rx GFP marking PB granulocytes, %/mononuclear fraction, % . | DHFR vector copy no./cells × 10-2 . | Peak GFP marking PB granulocytes, %/mononuclear fractions, % . | Days until decay to baseline . | Toxicity . | |||
| RC701 | VSV-G, MYFP-ir-DHFR2 | Rx 1, d 137 | T6 × 5, N3 × 5 | No | 0 | 0.3/ND | — | 1.0/ND | 15 | None | |||
| Rx 2, d 174 | T6 × 6, T8 × 1, N2.5 × 7 | No | 1 | ND/ND | — | ND/ND | NA | None | |||||
| Rx 3, d 210 | T6 × 2, T8 × 5, N3 × 7 | No | 3 | 0.3/ND | — | 1.3/ND | 24 | None | |||||
| RQ2278 | RD114, MgirL22Y | Rx 1, d 80 | T6 × 2, T8 × 5, N3 × 7 | No | At least 3 | 1/3 | — | 12/19 | NA | Died day 9 after Rx, Gl bleeding, ANC 14, plts 24 000 | |||
| RQ2258 | RD114, MgirL22Y | Rx 1, d 109 | T6 × 5, N3 × 5 | No | 2 | 1/2 | — | 10/13 | 17 | Dehydration, fecal blood | |||
| Rx 2, d 193 | T6 × 5, N3 × 5 | Yes | At least 2 | 0.8/1.4 | — | 9/5 | NA | Died day 7 after Rx from staph sepsis (catheter) | |||||
| 96E113 | RD114, MgirL22Y | Rx 1, d 690 | T6 × 5, N3 × 5 | Yes | 3 | 2/2 | — | 19/60 | 21 | Dehydration, hypernatremia, hyperglycemia | |||
| RQ2282 | PG13, MDHFR2wc and ampho G1Na | Rx 1, d 424 | T6 × 5, N2 × 5 | Yes | 1 | — | 0.8/0.9 | No change | NA | None | |||
| Rx 2, d 480 | T8 × 5, N2 × 5 | Yes | 4 | — | 0.8/1.0 | Increased DNA copy no. | NA | None | |||||
| RQ2788 | PG13, MDHFR2wc and ampho G1Na | Rx 1, d 207 | T6 × 5, N2.5 × 5 | Yes | 2 | — | 0.5/0.35 | Increased DNA copy no. | Gl toxicity | ||||
. | . | Drug treatment . | . | . | . | . | . | . | . | . | |||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Monkey . | Vector . | Day after transplantation . | Drug doses, mg/kg × d . | SCF + G-CSF before treatment . | Days ANC was below 500 . | Pre-Rx GFP marking PB granulocytes, %/mononuclear fraction, % . | DHFR vector copy no./cells × 10-2 . | Peak GFP marking PB granulocytes, %/mononuclear fractions, % . | Days until decay to baseline . | Toxicity . | |||
| RC701 | VSV-G, MYFP-ir-DHFR2 | Rx 1, d 137 | T6 × 5, N3 × 5 | No | 0 | 0.3/ND | — | 1.0/ND | 15 | None | |||
| Rx 2, d 174 | T6 × 6, T8 × 1, N2.5 × 7 | No | 1 | ND/ND | — | ND/ND | NA | None | |||||
| Rx 3, d 210 | T6 × 2, T8 × 5, N3 × 7 | No | 3 | 0.3/ND | — | 1.3/ND | 24 | None | |||||
| RQ2278 | RD114, MgirL22Y | Rx 1, d 80 | T6 × 2, T8 × 5, N3 × 7 | No | At least 3 | 1/3 | — | 12/19 | NA | Died day 9 after Rx, Gl bleeding, ANC 14, plts 24 000 | |||
| RQ2258 | RD114, MgirL22Y | Rx 1, d 109 | T6 × 5, N3 × 5 | No | 2 | 1/2 | — | 10/13 | 17 | Dehydration, fecal blood | |||
| Rx 2, d 193 | T6 × 5, N3 × 5 | Yes | At least 2 | 0.8/1.4 | — | 9/5 | NA | Died day 7 after Rx from staph sepsis (catheter) | |||||
| 96E113 | RD114, MgirL22Y | Rx 1, d 690 | T6 × 5, N3 × 5 | Yes | 3 | 2/2 | — | 19/60 | 21 | Dehydration, hypernatremia, hyperglycemia | |||
| RQ2282 | PG13, MDHFR2wc and ampho G1Na | Rx 1, d 424 | T6 × 5, N2 × 5 | Yes | 1 | — | 0.8/0.9 | No change | NA | None | |||
| Rx 2, d 480 | T8 × 5, N2 × 5 | Yes | 4 | — | 0.8/1.0 | Increased DNA copy no. | NA | None | |||||
| RQ2788 | PG13, MDHFR2wc and ampho G1Na | Rx 1, d 207 | T6 × 5, N2.5 × 5 | Yes | 2 | — | 0.5/0.35 | Increased DNA copy no. | Gl toxicity | ||||
Rx indicates drug treatment; T6, TMTX 6 mg/kg per day; N3, NBMPR-P 3 mg/kg per day; ND, not determined; T8, TMTX 8 mg/kg; N2.5, NBMPR-P 2.5 mg/kg; NA, not applicable; plts, platelets; N2, NBMPR-R 2 mg/kg; ampho, amphotrophic.