Hematopoietic progenitors detected in fetal livers of knock-in embryos at E12.5
Genotype . | n . | BFU-Es . | CFU-GMs . | CFU-Mix's . | Total . |
|---|---|---|---|---|---|
| Wild-type | |||||
| +/+ | 11 | 5 ± 1 (1725) | 73 ± 20 (25 524) | 17 ± 3 (5635) | 103 ± 25 (36 450) |
| WT/WT | 11 | 7 ± 1 (1219) | 79 ± 19 (14 684) | 20 ± 4 (3706) | 110 ± 20 (20 347) |
| Δ446 mutant | |||||
| +/+ | 11 | 6 ± 2 (1693) | 75 ± 27 (23 464) | 17 ± 7 (5253) | 104 ± 35 (28 729) |
| Δ446/Δ446 | 11 | 9 ± 3 (1609) | 86 ± 38 (13 163) | 23 ± 10 (3503) | 123 ± 51 (19 112) |
Genotype . | n . | BFU-Es . | CFU-GMs . | CFU-Mix's . | Total . |
|---|---|---|---|---|---|
| Wild-type | |||||
| +/+ | 11 | 5 ± 1 (1725) | 73 ± 20 (25 524) | 17 ± 3 (5635) | 103 ± 25 (36 450) |
| WT/WT | 11 | 7 ± 1 (1219) | 79 ± 19 (14 684) | 20 ± 4 (3706) | 110 ± 20 (20 347) |
| Δ446 mutant | |||||
| +/+ | 11 | 6 ± 2 (1693) | 75 ± 27 (23 464) | 17 ± 7 (5253) | 104 ± 35 (28 729) |
| Δ446/Δ446 | 11 | 9 ± 3 (1609) | 86 ± 38 (13 163) | 23 ± 10 (3503) | 123 ± 51 (19 112) |
Numbers are mean ± SD per 5000 nucleated cells cultured. Mean numbers of cells per liver appear in parentheses. WT indicates wild-type AML1 cDNA knock-in; Δ446, Δ446 mutant AML1 CDNA knock-in; BFU-E, erythroid burst-forming unit; CFU-GM, granulocyte-macrophage colony-forming unit; and CFU-Mix, mixed-lineage colony-forming unit.