Induction of selected genes by IL-6 in INA-6 cells as determined by microarray analysis, real-time PCR, and RNA protection assay
. | Microarray . | . | . | Real-time PCR . | . | . | RNA protection assay . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene . | 1 h . | 4 h . | Perm . | 1 h . | 4 h . | Perm . | 1 h . | 4 h . | Perm . | ||||||
| junB* | 6.7 | 2.2 | 1.9 | 12.1 ± 3.1 | 3.7 ± 1.4 | 3.0 ± 1.6 | 7.5 ± 0.5 | 2.1 ± 0.1 | 2.0 ± 0.4 | ||||||
| SOCS3 | 24.3 | 3.7 | 4.0 | 24.7 ± 1.3 | 4.6 ± 1.7 | 3.0 ± 1.7 | 19.9 ± 6.9 | 3.2 ± 0.3 | 2.6 ± 0.4 | ||||||
| HB-EGF | 3.1 | 2.2 | 1.9 | 5.3 ± 0.7 | 3.2 ± 0.4 | 3.2 ± 0.8 | 4.8 ± 0.7 | 2.2 ± 0.1 | 2.9 ± 0.3 | ||||||
| Pim-1* | 2.4 | 1.0 | 1.1 | 8.2 ± 0.7 | 2.3 ± 0.3 | 1.5 ± 0.1 | ND | ND | ND | ||||||
| Bcl-3 | 1.9 | 1.7 | 1.5 | 8.3 ± 2.4 | 4.7 ± 1.9 | 5.0 ± 0.7 | ND | ND | ND | ||||||
| SGK | 3.7 | 4.3 | 4.4 | 12.1 ± 3.1 | ND | ND | 10.3 ± 2.9 | 6.5 ± 0.9 | 8.8 ± 1.0 | ||||||
| GADD45β | 3.2 | 2.0 | 1.3 | 6.0 ± 2.6 | ND | ND | 2.6 ± 0.3 | 1.3 ± 0.3 | 1.1 ± 0.6 | ||||||
| DEC1 | 3.7 | 2.1 | 2.0 | ND | ND | ND | 5.3 ± 1.9 | 2.7 ± 0.4 | 2.7 ± 0.4 | ||||||
| DEPP | 6.1 | 2.5 | 1.6 | ND | ND | ND | 7.4 ± 1.9 | 1.9 ± 0.6 | 2.0 ± 0.4 | ||||||
. | Microarray . | . | . | Real-time PCR . | . | . | RNA protection assay . | . | . | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene . | 1 h . | 4 h . | Perm . | 1 h . | 4 h . | Perm . | 1 h . | 4 h . | Perm . | ||||||
| junB* | 6.7 | 2.2 | 1.9 | 12.1 ± 3.1 | 3.7 ± 1.4 | 3.0 ± 1.6 | 7.5 ± 0.5 | 2.1 ± 0.1 | 2.0 ± 0.4 | ||||||
| SOCS3 | 24.3 | 3.7 | 4.0 | 24.7 ± 1.3 | 4.6 ± 1.7 | 3.0 ± 1.7 | 19.9 ± 6.9 | 3.2 ± 0.3 | 2.6 ± 0.4 | ||||||
| HB-EGF | 3.1 | 2.2 | 1.9 | 5.3 ± 0.7 | 3.2 ± 0.4 | 3.2 ± 0.8 | 4.8 ± 0.7 | 2.2 ± 0.1 | 2.9 ± 0.3 | ||||||
| Pim-1* | 2.4 | 1.0 | 1.1 | 8.2 ± 0.7 | 2.3 ± 0.3 | 1.5 ± 0.1 | ND | ND | ND | ||||||
| Bcl-3 | 1.9 | 1.7 | 1.5 | 8.3 ± 2.4 | 4.7 ± 1.9 | 5.0 ± 0.7 | ND | ND | ND | ||||||
| SGK | 3.7 | 4.3 | 4.4 | 12.1 ± 3.1 | ND | ND | 10.3 ± 2.9 | 6.5 ± 0.9 | 8.8 ± 1.0 | ||||||
| GADD45β | 3.2 | 2.0 | 1.3 | 6.0 ± 2.6 | ND | ND | 2.6 ± 0.3 | 1.3 ± 0.3 | 1.1 ± 0.6 | ||||||
| DEC1 | 3.7 | 2.1 | 2.0 | ND | ND | ND | 5.3 ± 1.9 | 2.7 ± 0.4 | 2.7 ± 0.4 | ||||||
| DEPP | 6.1 | 2.5 | 1.6 | ND | ND | ND | 7.4 ± 1.9 | 1.9 ± 0.6 | 2.0 ± 0.4 | ||||||
Real-time PCR data and RNA protection assay, expressed as mean fold induction ± standard deviation, were obtained from at least 3 independent experiments by using RNA isolated from INA-6 cells incubated for 1 hour, 4 hours, or permanently with IL-6, compared with IL-6-deprived cells. Microarray data represent the mean fold induction of 2 experiments. ND indicates not determined; Perm, permanent.
junB and Pim-1 both are represented by 2-probe sets on the microarray. The microarray data given are the mean of the 2-probe set data.