Pathologic analysis of chimeric mice
Mouse ID . | Donor Hb, % . | Cardiac vascular ectasia . | Pulmonary artery medial thickness, μ . | Pulmonary vascular ectasia . | Remote hepatic infarct . | Recent hepatic infarct . | Hepatocyte iron . | Hepatic Kupffer cell iron . | Glomerular hypertrophy . | Renal mesangial hypercellularity . | Renal tubular iron . | Splenic architectural disruption . | Ongoing multiorgan injury . | Spleen cellularity . |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
eGFP1 | 100 | 0 | 0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 |
eGFP2 | 100 | 0 | 0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
eGFP3 | 100 | 0 | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
eGFP4 | 100 | 0 | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Sickle 1 | 0 | 4 | 1.1 | 4 | 3 | 4 | 2 | 3 | 2 | 3 | 3 | 4 | 4 | 4 |
Sickle 2 | 0 | 4 | 2.7 | 4 | 4 | 4 | 2 | 4 | 2 | 3 | 4 | 4 | 4 | 4 |
Sickle3 | 0 | 3 | 4 | 4 | 3 | 2 | 1 | 3 | 3 | 3 | 4 | 4 | 2 | 4 |
Sickle4 | 0 | 4 | 2.7 | 4 | 3 | 3 | 2 | 4 | 2 | 3 | 4 | 4 | 4 | 4 |
62 | 100 | 2 | 1.6 | 1 | 0 | 0 | 0 | 0 | 2 | 3 | 2 | 0 | 2 | 0 |
63 | 100 | 0 | 0.8 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 0 | 3 |
65 | 100 | 0 | 2.7 | 3 | 0 | 0 | 0 | 1 | 4 | 2 | 2 | 0 | 0 | 0 |
66 | 100 | 1 | 2.1 | 2 | 0 | 2 | 1 | 1 | 2 | 3 | 2 | 1 | 3 | 2 |
68 | 76 | 3 | 2.4 | 0 | 1 | 1 | 1 | 2 | 3 | 3 | 2 | 2 | 2 | 3 |
70 | 90 | 3 | 2.7 | 3 | 0 | 0 | 1 | 3 | 2 | 3 | 2 | 1 | 0 | 4 |
72 | 85 | 0 | 1.3 | 0 | 4 | 2 | 0 | 1 | 1 | 3 | 1 | 1 | 3 | 4 |
1550 | 43 | 4 | 2.7 | 2 | 1 | 4 | 2 | 4 | 3 | 4 | 4 | 4 | 3 | 4 |
1582 | 17 | 4 | 4 | 2 | 4 | 4 | 2 | 4 | 4 | 4 | 4 | 1 | 3 | 4 |
1604 | 61 | 3 | 1.3 | 0 | 3 | 4 | 2 | 3 | 2 | 2 | 4 | 4 | 2 | 4 |
1630 | 79 | 2 | 1.3 | 0 | 3 | 3 | 1 | 2 | 0 | 1 | 4 | 2 | 2 | 4 |
1636 | 57.5 | 3 | 3.2 | 0 | 3 | 2 | 2 | 3 | 2 | 3 | 4 | 4 | 3 | 4 |
1661 | 88 | 2 | 2.7 | 3 | 0 | 2 | 1 | 2 | 1 | 1 | 3 | 1 | 3 | 4 |
1664 | 95 | 0 | 2.1 | 0 | 0 | 1 | 1 | 2 | 3 | 3 | 3 | 1 | 0 | 4 |
1710 | 89 | 0 | NA | NA | 0 | 1 | 2 | 2 | 1 | 1 | 2 | 3 | 1 | 4 |
1718 | 88 | 1 | 2.7 | 4 | 0 | 1 | 1 | 2 | 2 | 2 | 3 | 1 | 0 | 4 |
1725 | 85 | 3 | 1.9 | 3 | 0 | 2 | 1 | 2 | 1 | 2 | 3 | 1 | 3 | 4 |
1748 | 92 | 0 | 2.7 | 0 | 0 | 0 | 1 | 2 | 2 | 1 | 3 | 1 | 0 | 4 |
1749 | 84 | 2 | 2.7 | 2 | 0 | 2 | 1 | 3 | 3 | 1 | 4 | 1 | 3 | 3 |
1780 | 91 | 0 | 1.6 | 0 | 2 | 3 | 2 | 2 | 1 | 1 | 3 | 2 | 2 | 3 |
1790 | 87 | 0 | 1.3 | 0 | 4 | 1 | 0 | 1 | 3 | 4 | 2 | 1 | 0 | 3 |
1798 | 88 | 3 | 1.9 | 0 | 4 | 1 | 1 | 2 | 1 | 2 | 3 | 1 | 2 | 3 |
Mouse ID . | Donor Hb, % . | Cardiac vascular ectasia . | Pulmonary artery medial thickness, μ . | Pulmonary vascular ectasia . | Remote hepatic infarct . | Recent hepatic infarct . | Hepatocyte iron . | Hepatic Kupffer cell iron . | Glomerular hypertrophy . | Renal mesangial hypercellularity . | Renal tubular iron . | Splenic architectural disruption . | Ongoing multiorgan injury . | Spleen cellularity . |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
eGFP1 | 100 | 0 | 0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 |
eGFP2 | 100 | 0 | 0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
eGFP3 | 100 | 0 | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
eGFP4 | 100 | 0 | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 |
Sickle 1 | 0 | 4 | 1.1 | 4 | 3 | 4 | 2 | 3 | 2 | 3 | 3 | 4 | 4 | 4 |
Sickle 2 | 0 | 4 | 2.7 | 4 | 4 | 4 | 2 | 4 | 2 | 3 | 4 | 4 | 4 | 4 |
Sickle3 | 0 | 3 | 4 | 4 | 3 | 2 | 1 | 3 | 3 | 3 | 4 | 4 | 2 | 4 |
Sickle4 | 0 | 4 | 2.7 | 4 | 3 | 3 | 2 | 4 | 2 | 3 | 4 | 4 | 4 | 4 |
62 | 100 | 2 | 1.6 | 1 | 0 | 0 | 0 | 0 | 2 | 3 | 2 | 0 | 2 | 0 |
63 | 100 | 0 | 0.8 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 0 | 3 |
65 | 100 | 0 | 2.7 | 3 | 0 | 0 | 0 | 1 | 4 | 2 | 2 | 0 | 0 | 0 |
66 | 100 | 1 | 2.1 | 2 | 0 | 2 | 1 | 1 | 2 | 3 | 2 | 1 | 3 | 2 |
68 | 76 | 3 | 2.4 | 0 | 1 | 1 | 1 | 2 | 3 | 3 | 2 | 2 | 2 | 3 |
70 | 90 | 3 | 2.7 | 3 | 0 | 0 | 1 | 3 | 2 | 3 | 2 | 1 | 0 | 4 |
72 | 85 | 0 | 1.3 | 0 | 4 | 2 | 0 | 1 | 1 | 3 | 1 | 1 | 3 | 4 |
1550 | 43 | 4 | 2.7 | 2 | 1 | 4 | 2 | 4 | 3 | 4 | 4 | 4 | 3 | 4 |
1582 | 17 | 4 | 4 | 2 | 4 | 4 | 2 | 4 | 4 | 4 | 4 | 1 | 3 | 4 |
1604 | 61 | 3 | 1.3 | 0 | 3 | 4 | 2 | 3 | 2 | 2 | 4 | 4 | 2 | 4 |
1630 | 79 | 2 | 1.3 | 0 | 3 | 3 | 1 | 2 | 0 | 1 | 4 | 2 | 2 | 4 |
1636 | 57.5 | 3 | 3.2 | 0 | 3 | 2 | 2 | 3 | 2 | 3 | 4 | 4 | 3 | 4 |
1661 | 88 | 2 | 2.7 | 3 | 0 | 2 | 1 | 2 | 1 | 1 | 3 | 1 | 3 | 4 |
1664 | 95 | 0 | 2.1 | 0 | 0 | 1 | 1 | 2 | 3 | 3 | 3 | 1 | 0 | 4 |
1710 | 89 | 0 | NA | NA | 0 | 1 | 2 | 2 | 1 | 1 | 2 | 3 | 1 | 4 |
1718 | 88 | 1 | 2.7 | 4 | 0 | 1 | 1 | 2 | 2 | 2 | 3 | 1 | 0 | 4 |
1725 | 85 | 3 | 1.9 | 3 | 0 | 2 | 1 | 2 | 1 | 2 | 3 | 1 | 3 | 4 |
1748 | 92 | 0 | 2.7 | 0 | 0 | 0 | 1 | 2 | 2 | 1 | 3 | 1 | 0 | 4 |
1749 | 84 | 2 | 2.7 | 2 | 0 | 2 | 1 | 3 | 3 | 1 | 4 | 1 | 3 | 3 |
1780 | 91 | 0 | 1.6 | 0 | 2 | 3 | 2 | 2 | 1 | 1 | 3 | 2 | 2 | 3 |
1790 | 87 | 0 | 1.3 | 0 | 4 | 1 | 0 | 1 | 3 | 4 | 2 | 1 | 0 | 3 |
1798 | 88 | 3 | 1.9 | 0 | 4 | 1 | 1 | 2 | 1 | 2 | 3 | 1 | 2 | 3 |
Mice were immersion fixed after death, sectioned, stained, and analyzed in a blinded manner by a single pathologist. A pathologic score of 0 (no sickle pathology) to 4 (classic sickle pathology) was assigned to the indicated characteristics. Mouse ID = internal identifier. eGFP1-4 refer to control mice; Sickle 1-4, untransplanted sickle mice; and 62-1798, transplanted sickle mice. NA indicates not assessed.