Oligonucleotides and probes for TREC quantification
DNA circles quantification | ||||
| sjTREC | 5′ -In: | GCTCTGAAAGGCAGAAAGAGG | ||
| 5′ -Out: | CTCTCCTATCTCTGCTCTGAA | |||
| 3′ -In: | ACATTTGCTCCGTGGTCTGTG | |||
| 3′ -Out: | ACTCACTTTTCCGAGGCTGA | |||
| Dβ1 | 3′ -In: | TGTGACCCAGGAGGAAAGAAG | ||
| 3′ -Out: | CTCATCTGGGCCTGTCCTTGT | |||
| Jβ1S1 | 5′ -In: | CCCTCTCTATGCCTTCAATGT | ||
| 5′ -Out: | GAACCTAGGACCCTGTGGA | |||
| Jβ1S2 | 5′ -In: | CAGATCCGTCACAGGGAAAAGT | ||
| 5′ -Out: | ACAAGGCACCAGACTCACAGTT | |||
| Jβ1S3 | 5′ -In: | TGTCCCTGTGAGGGAAGAGTT | ||
| 5′ -Out: | AAGGGAACACAGAGTACTGGAA | |||
| Jβ1S4 | 5′ -In: | TGGACTTGGGGAGGCAGGA | ||
| 5′ -Out: | GGATCACACGGGGCCTAATT | |||
| Jβ1S5 | 5′ -In: | CTCATAAAATGTGGGTCAGTGGA | ||
| 5′ -Out: | GAAACTGAGAACACAGCCAAGAA | |||
| Jβ1S6 | 5′ -In: | TGAATCCAGGCAGAGAAAGG | ||
| 5′ -Out: | ATCCTCCCTCTTATGTGCATGG | |||
| CD3γ | 5′ -In: | TGGCTGTCCTCATCCTGG | ||
| 5′ -Out: | ACTGACATGGAACAGGGGAAG | |||
| 3′ -In: | CTTGGCCTATGCCCTTTTGG | |||
| 3′ -Out: | CCAGCTCTGAAGTAGGGAACATAT | |||
| LightCycler probes | ||||
| Sj | P1: | AATAAGTTCAGCCCTCCATGTCACACTf | ||
| P2: | (Red! 640) TGTTTTCCATCCTGGGGAGTGTTTCAp | |||
| Dβ1 | P1: | CTGGGAGTTGGGACCGCCAGAGAGGTf | ||
| P2: | (Red! 640) TTTGTAAAGGTTTCCCGTAGAGTTGAATCATTGTp | |||
| CD3γ | P1: | GGCTGAAGGTTAGGGATACCAATATTCCTGTCTCf | ||
| P2: | (Red! 705) CTAGTGATGGGCTCTTCCCTTGAGCCCTTCp | |||
DNA circles quantification | ||||
| sjTREC | 5′ -In: | GCTCTGAAAGGCAGAAAGAGG | ||
| 5′ -Out: | CTCTCCTATCTCTGCTCTGAA | |||
| 3′ -In: | ACATTTGCTCCGTGGTCTGTG | |||
| 3′ -Out: | ACTCACTTTTCCGAGGCTGA | |||
| Dβ1 | 3′ -In: | TGTGACCCAGGAGGAAAGAAG | ||
| 3′ -Out: | CTCATCTGGGCCTGTCCTTGT | |||
| Jβ1S1 | 5′ -In: | CCCTCTCTATGCCTTCAATGT | ||
| 5′ -Out: | GAACCTAGGACCCTGTGGA | |||
| Jβ1S2 | 5′ -In: | CAGATCCGTCACAGGGAAAAGT | ||
| 5′ -Out: | ACAAGGCACCAGACTCACAGTT | |||
| Jβ1S3 | 5′ -In: | TGTCCCTGTGAGGGAAGAGTT | ||
| 5′ -Out: | AAGGGAACACAGAGTACTGGAA | |||
| Jβ1S4 | 5′ -In: | TGGACTTGGGGAGGCAGGA | ||
| 5′ -Out: | GGATCACACGGGGCCTAATT | |||
| Jβ1S5 | 5′ -In: | CTCATAAAATGTGGGTCAGTGGA | ||
| 5′ -Out: | GAAACTGAGAACACAGCCAAGAA | |||
| Jβ1S6 | 5′ -In: | TGAATCCAGGCAGAGAAAGG | ||
| 5′ -Out: | ATCCTCCCTCTTATGTGCATGG | |||
| CD3γ | 5′ -In: | TGGCTGTCCTCATCCTGG | ||
| 5′ -Out: | ACTGACATGGAACAGGGGAAG | |||
| 3′ -In: | CTTGGCCTATGCCCTTTTGG | |||
| 3′ -Out: | CCAGCTCTGAAGTAGGGAACATAT | |||
| LightCycler probes | ||||
| Sj | P1: | AATAAGTTCAGCCCTCCATGTCACACTf | ||
| P2: | (Red! 640) TGTTTTCCATCCTGGGGAGTGTTTCAp | |||
| Dβ1 | P1: | CTGGGAGTTGGGACCGCCAGAGAGGTf | ||
| P2: | (Red! 640) TTTGTAAAGGTTTCCCGTAGAGTTGAATCATTGTp | |||
| CD3γ | P1: | GGCTGAAGGTTAGGGATACCAATATTCCTGTCTCf | ||
| P2: | (Red! 705) CTAGTGATGGGCTCTTCCCTTGAGCCCTTCp | |||