Determination of the origin (donor versus recipient) of peptide-specific CD8+ T-cell clones by PCR analysis of sex chromosome or informative SNPs
Study no. . | Sample . | Sex/informative SNPs . |
|---|---|---|
| 01 | ||
| Recipient | PBMC (before alloSCT) | MTHFR C677C/C677T |
| Donor | PBMC | MTHFR C677C/C677C |
| Recipient | Clone 1.3 (after alloSCT) | MTHFR C677C/C677C |
| Recipient | Clone 1.4 (after alloSCT) | MTHFR C677C/C677C |
| 02 | ||
| Recipient | PBMC (before alloSCT) | XX |
| Donor | PBMC | XY |
| Recipient | Clone 2.2 (after alloSCT) | XY |
| Recipient | Clone 2.3 (after alloSCT) | MTHFR C677C/C677C |
| 03 | ||
| Recipient | PBMC (before alloSCT) | XY |
| Donor | PBMC | XY |
| Recipient | Clone 3.2a (after alloSCT) | XX |
| Recipient | Clone 3.2b (after alloSCT) | XX |
| 04 | ||
| Recipient | PBMC (before alloSCT) | ApoB: EcoR1 G4154G/G4154A; Xba1 C2488T/C2488T; Msp1 A3611A/A3611A |
| Donor | PBMC | ApoB: EcoR1 G4154G/G4154G; Xba1 C2488C/C2488C; Msp1 A3611A/A3611G |
| Recipient | Clone 4.2 (after alloSCT) | ApoB: EcoR1 G4154G/G4154G; Xba1 C2488C/C2488C; Msp1 A3611A/A3611G |
| 05 | ||
| Recipient | PBMC (before alloSCT) | HPA 3a/3a; HPA 5a/5a |
| Donor | PBMC | HPA 3a/3b; HPA 5a/5b |
| Recipient | Clone 5.2 (after alloSCT) | HPA 3a/3b; HPA 5a/5b |
| 06 | ||
| Recipient | Buccal scrape (after alloSCT) | TNF G-308G/G-308G |
| Donor | PBMC | TNF G-308G/G-308A |
| Recipient | Clone 6.2 (after alloSCT) | TNF G-308G/G-308A |
| 09 | ||
| Recipient | PBMC (before alloSCT) | XX |
| Donor | PBMC | XY |
| Recipient | Clone 9.1 (after alloSCT) | XY |
Study no. . | Sample . | Sex/informative SNPs . |
|---|---|---|
| 01 | ||
| Recipient | PBMC (before alloSCT) | MTHFR C677C/C677T |
| Donor | PBMC | MTHFR C677C/C677C |
| Recipient | Clone 1.3 (after alloSCT) | MTHFR C677C/C677C |
| Recipient | Clone 1.4 (after alloSCT) | MTHFR C677C/C677C |
| 02 | ||
| Recipient | PBMC (before alloSCT) | XX |
| Donor | PBMC | XY |
| Recipient | Clone 2.2 (after alloSCT) | XY |
| Recipient | Clone 2.3 (after alloSCT) | MTHFR C677C/C677C |
| 03 | ||
| Recipient | PBMC (before alloSCT) | XY |
| Donor | PBMC | XY |
| Recipient | Clone 3.2a (after alloSCT) | XX |
| Recipient | Clone 3.2b (after alloSCT) | XX |
| 04 | ||
| Recipient | PBMC (before alloSCT) | ApoB: EcoR1 G4154G/G4154A; Xba1 C2488T/C2488T; Msp1 A3611A/A3611A |
| Donor | PBMC | ApoB: EcoR1 G4154G/G4154G; Xba1 C2488C/C2488C; Msp1 A3611A/A3611G |
| Recipient | Clone 4.2 (after alloSCT) | ApoB: EcoR1 G4154G/G4154G; Xba1 C2488C/C2488C; Msp1 A3611A/A3611G |
| 05 | ||
| Recipient | PBMC (before alloSCT) | HPA 3a/3a; HPA 5a/5a |
| Donor | PBMC | HPA 3a/3b; HPA 5a/5b |
| Recipient | Clone 5.2 (after alloSCT) | HPA 3a/3b; HPA 5a/5b |
| 06 | ||
| Recipient | Buccal scrape (after alloSCT) | TNF G-308G/G-308G |
| Donor | PBMC | TNF G-308G/G-308A |
| Recipient | Clone 6.2 (after alloSCT) | TNF G-308G/G-308A |
| 09 | ||
| Recipient | PBMC (before alloSCT) | XX |
| Donor | PBMC | XY |
| Recipient | Clone 9.1 (after alloSCT) | XY |
All clones were obtained in the recipient following alloSCT contained donor DNA.
SNP indicated single nucleotide polymorphism; MTHFR, methyl-tetra-hydrofolate reductase; ApoB, apolipoprotein B; HPA, human platelet antigen; TNF, tumor necrosis factor α.