Quantitation of AID+ fraction of low AID-expressing samples
. | Cells/well . | . | . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Sample . | 400 . | 800 . | 1600 . | 3200 . | 6400 . | AID+ fraction, % . | QPCR value* . | ||||
| CLL203 | 1/93 | — | 2/92 | — | — | <0.0048 | 0.28 | ||||
| CLL127 | — | 0/32 | 1/32 | 0/32 | — | <0.0031 | 0.50 | ||||
| CLL400 | — | 0/28 | 0/29 | 0/25 | — | <0.0024 | 0.18 | ||||
| CLL361 | — | 1/32 | 0/32 | 1/31 | — | <0.0058 | 5.20 | ||||
| CLL156 | — | 0/32 | 0/32 | — | 1/28 | <0.0022 | 1.27 | ||||
| Normal A | 0/31 | — | 0/31 | — | 0/31 | <0.0014 | — | ||||
| Normal B | 0/32 | — | 0/32 | — | 0/30 | <0.0008 | — | ||||
. | Cells/well . | . | . | . | . | . | . | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Sample . | 400 . | 800 . | 1600 . | 3200 . | 6400 . | AID+ fraction, % . | QPCR value* . | ||||
| CLL203 | 1/93 | — | 2/92 | — | — | <0.0048 | 0.28 | ||||
| CLL127 | — | 0/32 | 1/32 | 0/32 | — | <0.0031 | 0.50 | ||||
| CLL400 | — | 0/28 | 0/29 | 0/25 | — | <0.0024 | 0.18 | ||||
| CLL361 | — | 1/32 | 0/32 | 1/31 | — | <0.0058 | 5.20 | ||||
| CLL156 | — | 0/32 | 0/32 | — | 1/28 | <0.0022 | 1.27 | ||||
| Normal A | 0/31 | — | 0/31 | — | 0/31 | <0.0014 | — | ||||
| Normal B | 0/32 | — | 0/32 | — | 0/30 | <0.0008 | — | ||||
Summary of the results of the limiting dilution experiments performed on 8 CLL cases and 2 elderly healthy individuals (Figure 4A-B). The results are shown as the ratio of the AID+ wells to VH-positive wells at indicated dilutions for each sample. In the last 2 columns on the right, the AID+ fractions are expressed as a percentage, and the real time quantitative PCR values are reported for each sample, respectively. If there were insufficient numbers of positive cells to properly interpolate as in Figure 4B, a Poisson distribution was used to establish the upper bound of the 95% confidence interval. — indicates that no experiment was performed at those conditions.
Expression units, as per Figure 2.