Characterization of the reactive oxygen species (ROS) produced by leukocytes in the presence of anti-D–opsonized RBCs
. | . | Monocytes . | . | Granulocytes . | . | ||
|---|---|---|---|---|---|---|---|
| Fluorescent dye . | ROS detected . | Fold change . | P . | Fold change . | P . | ||
| DHR 123 | H2O2, ONOO-, HOCI | 6.71 ± 5.11 | .005 | 61.80 ± 11.56 | .0001 | ||
| DAF-2-DA | NO | 0.57 ± 0.14 | NS | 1.33 ± 0.68 | NS | ||
| TEMPO-9-AC | O2-, OH | 5.68 ± 0.93 | .006 | 9.58 ± 0.75 | .0007 | ||
. | . | Monocytes . | . | Granulocytes . | . | ||
|---|---|---|---|---|---|---|---|
| Fluorescent dye . | ROS detected . | Fold change . | P . | Fold change . | P . | ||
| DHR 123 | H2O2, ONOO-, HOCI | 6.71 ± 5.11 | .005 | 61.80 ± 11.56 | .0001 | ||
| DAF-2-DA | NO | 0.57 ± 0.14 | NS | 1.33 ± 0.68 | NS | ||
| TEMPO-9-AC | O2-, OH | 5.68 ± 0.93 | .006 | 9.58 ± 0.75 | .0007 | ||
Identification of reactive oxygen species (ROS) and reactive nitrogen intermediates (RNIs) using dihydrorhodamine 123 (DHR 123), 4,5-diaminofluorescein diacetate (DAF-2-DA) and 4-((9-acridinecarbonyl)amino)-2,2,6,6-tetramethylpiperidin-1-oxyl (TEMPO-9-AC). The method used was the same as that described in the legend of Figure 1, except for the substitution of DHR 123 with the various fluorescent dyes. Each fluorophore was used at a final concentration of 10 μM, and the phagocytic burst shown is at a 2000:1 (RBC/WBC) ratio. Data are expressed as mean ± SD. NS indicates not significant.