Specificity constants for normal and BβArg14His fibrinogen with thrombin and batroxobin
. | Thrombin* . | . | Batroxobin† . | |
|---|---|---|---|---|
. | kcat/Km, FpA . | kcat/Km, FpB . | kcat/Km, FpA . | |
| Normal | 14.3 ± 9.2 | 3.1 ± 1.1 | 1.12 ± 0.26 | |
| BβArg14His | 13.5 ± 7.5 | 0.011 ± 0.002 | 1.45 ± 0.64 | |
. | Thrombin* . | . | Batroxobin† . | |
|---|---|---|---|---|
. | kcat/Km, FpA . | kcat/Km, FpB . | kcat/Km, FpA . | |
| Normal | 14.3 ± 9.2 | 3.1 ± 1.1 | 1.12 ± 0.26 | |
| BβArg14His | 13.5 ± 7.5 | 0.011 ± 0.002 | 1.45 ± 0.64 | |
Values are the averages from 4 experiments-all with 0.1 mg/mL fibrinogen and 1 with 0.005 U/mL thrombin, 1 with 0.25 U/mL thrombin, and 2 with 0.1 U/mL thrombin-and are expressed as 106 M−1 sec−1.
Values are the averages from 2 experiments with 0.005 U/mL batroxobin and are expressed as 106 M−1 sec−1.