VH and VL sequence analysis of IgM aCL (MVH and MVL) originating from single cell sorting (patient 1)
. | Vhfamily . | Vkfamily . | Germline gene . | Homology (%)* . | R/S CDR . | R/S FR . | D gene . | Jhgene . | Jkgene . |
|---|---|---|---|---|---|---|---|---|---|
| MVH1 | 1 | — | V1-2 | 100 | — | — | D2-2 | 4 | — |
| D3-3 | |||||||||
| MVH2 | 3 | — | V3-48 | 96.3 | 1/1 | 6/1 | D3-16 | 4 | — |
| MVH3 | 1 | — | V1-2 | 99.7 | — | 0/1 | D3-22/in† | 4 | — |
| MVH4 | 4 | — | V4-b | 100 | — | — | D5-5 | 4 | — |
| MVH5 | 3 | — | V3-13 | 98 | 1/0 | 2/0 | D3-10 | 6 | — |
| P = .329‡ | |||||||||
| MVH6 | 3 | — | V3-21 | 98.7 | 1/0 | 1/1 | D5-5 | 4 | — |
| P = .335 | |||||||||
| MVH7 | 2 | — | V2-5 | 100 | — | — | D3-3 | 5 | — |
| MVH8 | 1 | — | V1-3 | 90.7 | 7/1 | 7/8 | D3-10 | 4 | — |
| P = .0375 | |||||||||
| MVH9 | 3 | — | V3-48 | 96.8 | 4/1 | 0/2 | D1-26 | 4 | — |
| P = .0116 | |||||||||
| MVL1 | — | 2 | V2-28 | 100 | — | — | — | — | 1 |
| MVL2 | — | 1 | V1-39 | 99.7 | — | 1/0 | — | — | 3 |
| MVL3 | — | 4 | V4-1 | 100 | — | — | — | — | 2 |
| MVL4 | — | 1 | V1-13 | 100 | — | — | — | — | 5 |
| MVL5 | — | 1 | V1-39 | 100 | — | — | — | — | 3 |
| MVL6 | — | 4 | V4-1 | 99.3 | — | 1/0 | — | — | 1 |
| MVL7 | — | 3 | V3-20 | 99.6 | — | 0/1 | — | — | 1 |
| MVL8 | — | 1 | V1-5 | 97 | 3/1 | 2/2 | — | — | 2 |
| P = .0195 | |||||||||
| MVL9 | — | 1 | V1-8 | 100 | — | — | — | — | 4 |
. | Vhfamily . | Vkfamily . | Germline gene . | Homology (%)* . | R/S CDR . | R/S FR . | D gene . | Jhgene . | Jkgene . |
|---|---|---|---|---|---|---|---|---|---|
| MVH1 | 1 | — | V1-2 | 100 | — | — | D2-2 | 4 | — |
| D3-3 | |||||||||
| MVH2 | 3 | — | V3-48 | 96.3 | 1/1 | 6/1 | D3-16 | 4 | — |
| MVH3 | 1 | — | V1-2 | 99.7 | — | 0/1 | D3-22/in† | 4 | — |
| MVH4 | 4 | — | V4-b | 100 | — | — | D5-5 | 4 | — |
| MVH5 | 3 | — | V3-13 | 98 | 1/0 | 2/0 | D3-10 | 6 | — |
| P = .329‡ | |||||||||
| MVH6 | 3 | — | V3-21 | 98.7 | 1/0 | 1/1 | D5-5 | 4 | — |
| P = .335 | |||||||||
| MVH7 | 2 | — | V2-5 | 100 | — | — | D3-3 | 5 | — |
| MVH8 | 1 | — | V1-3 | 90.7 | 7/1 | 7/8 | D3-10 | 4 | — |
| P = .0375 | |||||||||
| MVH9 | 3 | — | V3-48 | 96.8 | 4/1 | 0/2 | D1-26 | 4 | — |
| P = .0116 | |||||||||
| MVL1 | — | 2 | V2-28 | 100 | — | — | — | — | 1 |
| MVL2 | — | 1 | V1-39 | 99.7 | — | 1/0 | — | — | 3 |
| MVL3 | — | 4 | V4-1 | 100 | — | — | — | — | 2 |
| MVL4 | — | 1 | V1-13 | 100 | — | — | — | — | 5 |
| MVL5 | — | 1 | V1-39 | 100 | — | — | — | — | 3 |
| MVL6 | — | 4 | V4-1 | 99.3 | — | 1/0 | — | — | 1 |
| MVL7 | — | 3 | V3-20 | 99.6 | — | 0/1 | — | — | 1 |
| MVL8 | — | 1 | V1-5 | 97 | 3/1 | 2/2 | — | — | 2 |
| P = .0195 | |||||||||
| MVL9 | — | 1 | V1-8 | 100 | — | — | — | — | 4 |
R/S indicates the ratio of replacement mutations (R) and silent mutations (S) in the complementary-determining regions (CDR1 and CDR2) and framework (FR1, FR2, and FR3).—indicates not applicable.
Homology % provides the percentage of nucleotide sequence homology between the Ig V gene and its putative germ line.
In indicates sequence inserted in inverted orientation.
P is the probability that an excess of R mutations in the CDRs resulted by chance.