γδ T-cell distribution in gastrointestinal mucosa and draining lymph nodes, tonsil, and spleen
. | . | Secondary follicles . | . | T zone . | |
|---|---|---|---|---|---|
| Tissue* . | No. samples†(no. donors) . | No. analyzed GCs, (range of no. of GCs per sample) . | γδ T-cell-positive GCs, %‡(range per sample) . | γδ T-cell density,§cells per mm2 ± SD . | |
| Mucosa | |||||
| Normal | 9 (5) | 70 (3-26) | 53 (0-85) | ND | |
| Yersinia | 14 (3) | 286 (4-42) | 48 (0-100) | ND | |
| Morbus Crohn | 3 (3) | 37 (8-18) | 37 (0-67) | ND | |
| LN | |||||
| Normal | 32 (7) | 847 (2-85) | 25 (0-88) | 75 ± 30 | |
| Yersinia | 12 (3) | 305 (2-87) | 72 (38-100) | 22 ± 5 | |
| Morbus Crohn | 2 (2) | 121 (60-61) | 63 (58-67) | 73 ± 6 | |
| Tonsil | |||||
| Tonsillectomy | 5 (5) | 172 (16-50) | 20 (0-59) | 57 ± 28 | |
| Spleen | |||||
| Normal | 3 (3) | 87 (0-87) | 24∥ | ND | |
. | . | Secondary follicles . | . | T zone . | |
|---|---|---|---|---|---|
| Tissue* . | No. samples†(no. donors) . | No. analyzed GCs, (range of no. of GCs per sample) . | γδ T-cell-positive GCs, %‡(range per sample) . | γδ T-cell density,§cells per mm2 ± SD . | |
| Mucosa | |||||
| Normal | 9 (5) | 70 (3-26) | 53 (0-85) | ND | |
| Yersinia | 14 (3) | 286 (4-42) | 48 (0-100) | ND | |
| Morbus Crohn | 3 (3) | 37 (8-18) | 37 (0-67) | ND | |
| LN | |||||
| Normal | 32 (7) | 847 (2-85) | 25 (0-88) | 75 ± 30 | |
| Yersinia | 12 (3) | 305 (2-87) | 72 (38-100) | 22 ± 5 | |
| Morbus Crohn | 2 (2) | 121 (60-61) | 63 (58-67) | 73 ± 6 | |
| Tonsil | |||||
| Tonsillectomy | 5 (5) | 172 (16-50) | 20 (0-59) | 57 ± 28 | |
| Spleen | |||||
| Normal | 3 (3) | 87 (0-87) | 24∥ | ND | |
ND indicates not determined.
Secondary follicles containing germinal centers (GCs) and T-zone areas of the listed samples were examined by immunohistochemistry using the A20 pan-VγVδ-TCR Ab.
No. samples refers to the number of individual lymphoid organs analyzed, which originated from the number of donors as indicated in parentheses.
The average of γδ T-cell-positive GCs in percent and the range in percent of γδ T cell-positive GCs within a tissue sample are indicated.
γδ T-cell density in the T zone refers to the number of γδ T cells per mm2 ± SD (5 × 0.31 mm2 areas per sample were counted).
Only 1 of 3 samples from 3 individual donors contained secondary follicles with germinal centers (GCs).