Partial inhibition of tubular formation by EP2 receptor antagonist
Stimulant . | Number of junctions, mean ± SEM . |
|---|---|
| bFGF | 107.3 ± 8.1 |
| bFGF + AH6809 | 72.0 ± 12.2 |
| VEGF | 112.3 ± 5.0 |
| VEGF + AH6809 | 79.0 ± 6.9 |
Stimulant . | Number of junctions, mean ± SEM . |
|---|---|
| bFGF | 107.3 ± 8.1 |
| bFGF + AH6809 | 72.0 ± 12.2 |
| VEGF | 112.3 ± 5.0 |
| VEGF + AH6809 | 79.0 ± 6.9 |
HMECs were plated on growth factor—depleted Matrigel and stimulated with VEGF (10 ng/mL) or bFGF (50 ng/mL) in the presence or absence of AH6809 (10 μM). Twenty hours after stimulation, the number of junctions was quantified in at least 5 different fields under each treatment. AH6809 inhibits tubular formation induced by bFGF (32%), VEGF (29%). P < .05 as compared with each growth factor without AH6809.