Peripheral blood counts and splenic flow cytometry on preleukemic and leukemic animals
. | . | Age, mo . | WBC count, × 106/mL . | . | PLT count, × 106/mL . | . | Spleen . | . | . | ||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Mouse . | Genotype . | . | . | %PMNs . | . | HCT . | % Gr-1+ . | % CD34+ . | % Gr-1+/%CD34+ . | ||
| Wild type | C57B1/6 | 1 | 4.24 | 16.78 | 306 | 23.1 | 8.68 | 0.89 | 0.48 | ||
| Wild type | C57B1/6 | 1 | 2.6 | 18.68 | 511 | 50.2 | 8.59 | 0.87 | 0.41 | ||
| Wild type | C57B1/6 | 2 | 4.38 | 10.62 | 608 | 48.4 | 8.18 | 1.99 | 0.94 | ||
| Wild type | C57B1/6 | 6 | 7.88 | 20.98 | 756 | 55.1 | 4.85 | 1.02 | 0.46 | ||
| Wild type | 129/SvJ | 3 | 4.22 | 23.33 | 452 | 69.1 | 9.42 | 1.52 | 0.71 | ||
| Wild type | 129/SvJ | 4 | 14.44 | 29.8 | 457 | 29.8 | 9.32 | 1.58 | 0.99 | ||
| Wild type | 129/SvJ | 6 | 7.74 | 20.7 | 284 | 36 | 6.41 | 1.06 | 0.47 | ||
| Average | 6.5 | 20.04 | 482 | 44.53 | 7.92 | 1.28 | 0.64 | ||||
| Nonleukemic | |||||||||||
| 17776 | +/PR | 1 | 6.42 | 21.46 | 647 | 41 | 18.47 | 8.33 | 4.39 | ||
| 17283 | +/PR | 3 | 4.86 | 21.38 | 953 | 44.3 | 20.01 | 6.04 | 4.22 | ||
| 16098 | +/PR | 6 | 5.62 | 11.79 | 354 | 21.2 | 19.23 | 3.92 | 2.7 | ||
| 15458 | +/PR | 10 | 7.72 | 12.6 | 481 | 27.4 | 22.25 | 5.62 | 3.77 | ||
| 14111 | +/PR | 10 | 9.4 | 37 | 2179 | 43.8 | 14.3 | 2.9 | 1.7 | ||
| 14142 | +/PR | 10 | 9.0 | 56 | 1230 | 46 | 11.2 | 6.3 | 2.6 | ||
| 14143 | +/PR | 10 | 13.2 | 34 | 1163 | 42.5 | 13.5 | 10.8 | 7.48 | ||
| 14586 | +/PR | 9 | 6.7 | 25 | 1124 | 45.2 | 11.6 | 3.42 | 1.9 | ||
| 14584 | +/PR | 9 | 6.7 | 12 | 954 | 46.7 | 12.5 | 5.8 | 2.5 | ||
| Average | 7.74 | 25.69 | 1009 | 39.79 | 15.9 | 5.9 | 3.47 | ||||
| Leukemic | |||||||||||
| 13646 | +/PR | 5 | 47 | 48 | 254 | 32.7 | 24.7 | 26 | 25.9 | ||
| 13843 | +/PR | 5 | 45.2 | 64 | 362 | 27.9 | 73.4 | 26.6 | 26.4 | ||
| 13487 | +/PR | 6 | 25.8 | 44 | 135 | 24.1 | 88 | 61.1 | 61 | ||
| 13498 | +/PR | 6 | 126 | 77 | 184 | 26 | 54.4 | 55.4 | 30.5 | ||
| 13499 | +/PR | 6 | 69 | 33 | 246 | 21.7 | 60.2 | 53 | 32.6 | ||
| 13659 | +/PR | 7 | 29.8 | 35 | 368 | 30.3 | 87 | 33 | 32.9 | ||
| 13346 | +/PR | 8 | 17 | 34.5 | 328 | 34.5 | 76.5 | 23.3 | 24.7 | ||
| 13437 | +/PR | 8 | 23.7 | 23 | 414 | 30.9 | 85.6 | 52.4 | 51.2 | ||
| 13355 | +/PR | 9 | 18.8 | 40.3 | 322 | 32.8 | 86.1 | 42.2 | 40.3 | ||
| Average | 44.7 | 43.53 | 290 | 29.0 | 78.4 | 41.1 | 36.2 | ||||
| Leukemic | |||||||||||
| 13834 | PR/PR | 6 | 50 | 81 | 413 | 31 | 65.8 | 38.6 | 31 | ||
| 13445 | PR/PR | 7 | 61.4 | 15 | 358 | 26.1 | 77.9 | 59.6 | 48.1 | ||
| 13484 | PR/PR | 7 | 36.6 | 25 | 141 | 31.6 | 92.5 | 46.9 | 42.2 | ||
| 13647 | PR/PR | 7 | 48.4 | 50 | 361 | 31.7 | 89.9 | 64.4 | 64.2 | ||
| 13648 | PR/PR | 7 | 49.9 | 55.6 | 331 | 35.3 | 97.9 | 26 | 26 | ||
| 13663 | PR/PR | 7 | 84.2 | 51 | 98 | 32.4 | 91.9 | 45.4 | 45.2 | ||
| 13178 | PR/PR | 8 | 56 | 65 | 213 | 35.7 | 97 | 50.6 | 44.7 | ||
| 13451 | PR/PR | 8 | 34.6 | 39 | 282 | 35.8 | 84.9 | 83.6 | 75.6 | ||
| 13442 | PR/PR | 10 | 66 | 39 | 697 | 19.7 | 61 | 43.4 | 42.9 | ||
| 13349 | PR/PR | 11 | 38.2 | 30.5 | 529 | 36.5 | 85.2 | 54.7 | 53.9 | ||
| Average | 52.5 | 45.2 | 342 | 31.6 | 84.4 | 51.3 | 47.4 | ||||
. | . | Age, mo . | WBC count, × 106/mL . | . | PLT count, × 106/mL . | . | Spleen . | . | . | ||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Mouse . | Genotype . | . | . | %PMNs . | . | HCT . | % Gr-1+ . | % CD34+ . | % Gr-1+/%CD34+ . | ||
| Wild type | C57B1/6 | 1 | 4.24 | 16.78 | 306 | 23.1 | 8.68 | 0.89 | 0.48 | ||
| Wild type | C57B1/6 | 1 | 2.6 | 18.68 | 511 | 50.2 | 8.59 | 0.87 | 0.41 | ||
| Wild type | C57B1/6 | 2 | 4.38 | 10.62 | 608 | 48.4 | 8.18 | 1.99 | 0.94 | ||
| Wild type | C57B1/6 | 6 | 7.88 | 20.98 | 756 | 55.1 | 4.85 | 1.02 | 0.46 | ||
| Wild type | 129/SvJ | 3 | 4.22 | 23.33 | 452 | 69.1 | 9.42 | 1.52 | 0.71 | ||
| Wild type | 129/SvJ | 4 | 14.44 | 29.8 | 457 | 29.8 | 9.32 | 1.58 | 0.99 | ||
| Wild type | 129/SvJ | 6 | 7.74 | 20.7 | 284 | 36 | 6.41 | 1.06 | 0.47 | ||
| Average | 6.5 | 20.04 | 482 | 44.53 | 7.92 | 1.28 | 0.64 | ||||
| Nonleukemic | |||||||||||
| 17776 | +/PR | 1 | 6.42 | 21.46 | 647 | 41 | 18.47 | 8.33 | 4.39 | ||
| 17283 | +/PR | 3 | 4.86 | 21.38 | 953 | 44.3 | 20.01 | 6.04 | 4.22 | ||
| 16098 | +/PR | 6 | 5.62 | 11.79 | 354 | 21.2 | 19.23 | 3.92 | 2.7 | ||
| 15458 | +/PR | 10 | 7.72 | 12.6 | 481 | 27.4 | 22.25 | 5.62 | 3.77 | ||
| 14111 | +/PR | 10 | 9.4 | 37 | 2179 | 43.8 | 14.3 | 2.9 | 1.7 | ||
| 14142 | +/PR | 10 | 9.0 | 56 | 1230 | 46 | 11.2 | 6.3 | 2.6 | ||
| 14143 | +/PR | 10 | 13.2 | 34 | 1163 | 42.5 | 13.5 | 10.8 | 7.48 | ||
| 14586 | +/PR | 9 | 6.7 | 25 | 1124 | 45.2 | 11.6 | 3.42 | 1.9 | ||
| 14584 | +/PR | 9 | 6.7 | 12 | 954 | 46.7 | 12.5 | 5.8 | 2.5 | ||
| Average | 7.74 | 25.69 | 1009 | 39.79 | 15.9 | 5.9 | 3.47 | ||||
| Leukemic | |||||||||||
| 13646 | +/PR | 5 | 47 | 48 | 254 | 32.7 | 24.7 | 26 | 25.9 | ||
| 13843 | +/PR | 5 | 45.2 | 64 | 362 | 27.9 | 73.4 | 26.6 | 26.4 | ||
| 13487 | +/PR | 6 | 25.8 | 44 | 135 | 24.1 | 88 | 61.1 | 61 | ||
| 13498 | +/PR | 6 | 126 | 77 | 184 | 26 | 54.4 | 55.4 | 30.5 | ||
| 13499 | +/PR | 6 | 69 | 33 | 246 | 21.7 | 60.2 | 53 | 32.6 | ||
| 13659 | +/PR | 7 | 29.8 | 35 | 368 | 30.3 | 87 | 33 | 32.9 | ||
| 13346 | +/PR | 8 | 17 | 34.5 | 328 | 34.5 | 76.5 | 23.3 | 24.7 | ||
| 13437 | +/PR | 8 | 23.7 | 23 | 414 | 30.9 | 85.6 | 52.4 | 51.2 | ||
| 13355 | +/PR | 9 | 18.8 | 40.3 | 322 | 32.8 | 86.1 | 42.2 | 40.3 | ||
| Average | 44.7 | 43.53 | 290 | 29.0 | 78.4 | 41.1 | 36.2 | ||||
| Leukemic | |||||||||||
| 13834 | PR/PR | 6 | 50 | 81 | 413 | 31 | 65.8 | 38.6 | 31 | ||
| 13445 | PR/PR | 7 | 61.4 | 15 | 358 | 26.1 | 77.9 | 59.6 | 48.1 | ||
| 13484 | PR/PR | 7 | 36.6 | 25 | 141 | 31.6 | 92.5 | 46.9 | 42.2 | ||
| 13647 | PR/PR | 7 | 48.4 | 50 | 361 | 31.7 | 89.9 | 64.4 | 64.2 | ||
| 13648 | PR/PR | 7 | 49.9 | 55.6 | 331 | 35.3 | 97.9 | 26 | 26 | ||
| 13663 | PR/PR | 7 | 84.2 | 51 | 98 | 32.4 | 91.9 | 45.4 | 45.2 | ||
| 13178 | PR/PR | 8 | 56 | 65 | 213 | 35.7 | 97 | 50.6 | 44.7 | ||
| 13451 | PR/PR | 8 | 34.6 | 39 | 282 | 35.8 | 84.9 | 83.6 | 75.6 | ||
| 13442 | PR/PR | 10 | 66 | 39 | 697 | 19.7 | 61 | 43.4 | 42.9 | ||
| 13349 | PR/PR | 11 | 38.2 | 30.5 | 529 | 36.5 | 85.2 | 54.7 | 53.9 | ||
| Average | 52.5 | 45.2 | 342 | 31.6 | 84.4 | 51.3 | 47.4 | ||||
Peripheral blood white cell counts (WBC count), percent neutrophils (% PMNs), platelet count (PLT count), and hematocrit (HCT) were determined by automated counting for wild-type, nonleukemic mCG+/PR, leukemic mCG+/PR, and mCGPR/PR animals at the indicated ages. Spleens of the same animals were analyzed by flow cytometry for expression of the surface markers Gr-1 and CD34.